5NZS

The structure of the COPI coat leaf in complex with the ArfGAP2 uncoating factor

5NZS の概要

• エントリーDOI: 10.2210/pdb5nzs/pdb
• EMDBエントリー: 3721
• 分子名称: Coatomer subunit alpha, Coatomer subunit beta, Coatomer subunit beta', ... (8 entities in total)
• 機能のキーワード: copi, coated vesicles, arfgap2, transport protein
• 由来する生物種: Mus musculus (House Mouse); 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 765519.49

構造登録者

Dodonova, S.O.,Aderhold, P.,Kopp, J.,Ganeva, I.,Roehling, S.,Hagen, W.J.H.,Sinning, I.,Wieland, F.,Briggs, J.A.G. (登録日: 2017-05-15, 公開日: 2017-06-28, 最終更新日: 2024-07-10)

主引用文献

Dodonova, S.O.,Aderhold, P.,Kopp, J.,Ganeva, I.,Rohling, S.,Hagen, W.J.,Sinning, I.,Wieland, F.,Briggs, J.A.
9 angstrom structure of the COPI coat reveals that the Arf1 GTPase occupies two contrasting molecular environments.
Elife, 6:-, 2017

PubMed Abstract: COPI coated vesicles mediate trafficking within the Golgi apparatus and between the Golgi and the endoplasmic reticulum. Assembly of a COPI coated vesicle is initiated by the small GTPase Arf1 that recruits the coatomer complex to the membrane, triggering polymerization and budding. The vesicle uncoats before fusion with a target membrane. Coat components are structurally conserved between COPI and clathrin/adaptor proteins. Using cryo-electron tomography and subtomogram averaging, we determined the structure of the COPI coat assembled on membranes in vitro at 9 Å resolution. We also obtained a 2.57 Å resolution crystal structure of βδ-COP. By combining these structures we built a molecular model of the coat. We additionally determined the coat structure in the presence of ArfGAP proteins that regulate coat dissociation. We found that Arf1 occupies contrasting molecular environments within the coat, leading us to hypothesize that some Arf1 molecules may regulate vesicle assembly while others regulate coat disassembly.

PubMed: 28621666
DOI: 10.7554/eLife.26691

実験手法

ELECTRON MICROSCOPY (10.1 Å)