5NT7

Structure of the LOTUS domain of Oskar in complex with the C-terminal RecA-like domain of Vasa

5NT7 の概要

• エントリーDOI: 10.2210/pdb5nt7/pdb
• 分子名称: ATP-dependent RNA helicase vasa, isoform A, Maternal effect protein oskar (3 entities in total)
• 機能のキーワード: dead-box rna helicase, atpase, rna binding, regulator, stimulator, germ plasm, nuage, lotus, hydrolase- hydrolase regulator complex, hydrolase-hydrolase regulator complex, hydrolase/hydrolase regulator
• 由来する生物種: Drosophila melanogaster (Fruit fly); 詳細
• 細胞内の位置: Cytoplasm : P09052
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 60330.31

構造登録者

Jeske, M.,Ephrussi, A.,Mueller, C.W. (登録日: 2017-04-27, 公開日: 2017-05-17, 最終更新日: 2024-01-17)

主引用文献

Jeske, M.,Muller, C.W.,Ephrussi, A.
The LOTUS domain is a conserved DEAD-box RNA helicase regulator essential for the recruitment of Vasa to the germ plasm and nuage.
Genes Dev., 31:939-952, 2017

PubMed Abstract: DEAD-box RNA helicases play important roles in a wide range of metabolic processes. Regulatory proteins can stimulate or block the activity of DEAD-box helicases. Here, we show that LOTUS (Limkain, Oskar, and Tudor containing proteins 5 and 7) domains present in the germline proteins Oskar, TDRD5 (Tudor domain-containing 5), and TDRD7 bind and stimulate the germline-specific DEAD-box RNA helicase Vasa. Our crystal structure of the LOTUS domain of Oskar in complex with the C-terminal RecA-like domain of Vasa reveals that the LOTUS domain occupies a surface on a DEAD-box helicase not implicated previously in the regulation of the enzyme's activity. We show that, in vivo, the localization of Vasa to the nuage and germ plasm depends on its interaction with LOTUS domain proteins. The binding and stimulation of Vasa DEAD-box helicases by LOTUS domains are widely conserved.

PubMed: 28536148
DOI: 10.1101/gad.297051.117

実験手法

X-RAY DIFFRACTION (1.4 Å)