5NR6
NMR structure and 1H, 13C and 15N signal assignments for Dictyostelium discoidans MATB protein S71A mutant
Summary for 5NR6
| Entry DOI | 10.2210/pdb5nr6/pdb |
| NMR Information | BMRB: 34127 |
| Descriptor | MatB protein (1 entity in total) |
| Functional Keywords | mating type determination factor, dna binding protein, homeobox |
| Biological source | Dictyostelium discoideum (Slime mold) |
| Total number of polymer chains | 1 |
| Total formula weight | 12652.26 |
| Authors | Neuhaus, D.,Hedgethorne, K.,Yang, J.-C. (deposition date: 2017-04-22, release date: 2017-09-20, Last modification date: 2024-05-15) |
| Primary citation | Hedgethorne, K.,Eustermann, S.,Yang, J.C.,Ogden, T.E.H.,Neuhaus, D.,Bloomfield, G. Homeodomain-like DNA binding proteins control the haploid-to-diploid transition in Dictyostelium. Sci Adv, 3:e1602937-e1602937, 2017 Cited by PubMed Abstract: Homeodomain proteins control the developmental transition between the haploid and diploid phases in several eukaryotic lineages, but it is not known whether this regulatory mechanism reflects the ancestral condition or, instead, convergent evolution. We have characterized the mating-type locus of the amoebozoan , which encodes two pairs of small proteins that determine the three mating types of this species; none of these proteins display recognizable homology to known families. We report that the nuclear magnetic resonance structures of two of them, MatA and MatB, contain helix-turn-helix folds flanked by largely disordered amino- and carboxyl-terminal tails. This fold closely resembles that of homeodomain transcription factors, and, like those proteins, MatA and MatB each bind DNA characteristically using the third helix of their folded domains. By constructing chimeric versions containing parts of MatA and MatB, we demonstrate that the carboxyl-terminal tail, not the central DNA binding motif, confers mating specificity, providing mechanistic insight into how a third mating type might have originated. Finally, we show that these homeodomain-like proteins specify zygote function: Hemizygous diploids, formed in crosses between a wild-type strain and a null mutant, grow and differentiate identically to haploids. We propose that MatA and MatB are divergent homeodomain proteins with a conserved function in triggering the haploid-to-diploid transition that can be traced back to the last common ancestor of eukaryotes. PubMed: 28879231DOI: 10.1126/sciadv.1602937 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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