5NPU

Inferred ancestral pyruvate decarboxylase

5NPU の概要

• エントリーDOI: 10.2210/pdb5npu/pdb
• 分子名称: ANC27, MAGNESIUM ION, THIAMINE DIPHOSPHATE, ... (5 entities in total)
• 機能のキーワード: pyruvate decarboxylase, lyase, ancestral sequence reconstruction
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 244708.20

構造登録者

Buddrus, L.,Crennell, S.J.,Leak, D.J.,Danson, M.J.,Andrews, E.S.V.,Arcus, V.L. (登録日: 2017-04-19, 公開日: 2018-03-07, 最終更新日: 2024-01-17)

主引用文献

Buddrus, L.,Andrews, E.S.V.,Leak, D.J.,Danson, M.J.,Arcus, V.L.,Crennell, S.J.
Crystal structure of an inferred ancestral bacterial pyruvate decarboxylase.
Acta Crystallogr F Struct Biol Commun, 74:179-186, 2018

PubMed Abstract: Pyruvate decarboxylase (PDC; EC 4.1.1.1) is a key enzyme in homofermentative metabolism where ethanol is the major product. PDCs are thiamine pyrophosphate- and Mg ion-dependent enzymes that catalyse the non-oxidative decarboxylation of pyruvate to acetaldehyde and carbon dioxide. As this enzyme class is rare in bacteria, current knowledge of bacterial PDCs is extremely limited. One approach to further the understanding of bacterial PDCs is to exploit the diversity provided by evolution. Ancestral sequence reconstruction (ASR) is a method of computational molecular evolution to infer extinct ancestral protein sequences, which can then be synthesized and experimentally characterized. Through ASR a novel PDC was generated, designated ANC27, that shares only 78% amino-acid sequence identity with its closest extant homologue (Komagataeibacter medellinensis PDC, GenBank accession No. WP_014105323.1), yet is fully functional. Crystals of this PDC diffracted to 3.5 Å resolution. The data were merged in space group P321, with unit-cell parameters a = b = 108.33, c = 322.65 Å, and contained two dimers (two tetramer halves) in the asymmetric unit. The structure was solved by molecular replacement using PDB entry 2wvg as a model, and the final R values were R = 0.246 (0.3671 in the highest resolution bin) and R = 0.319 (0.4482 in the highest resolution bin). Comparison with extant bacterial PDCs supports the previously observed correlation between decreased tetramer interface area (and number of interactions) and decreased thermostability.

PubMed: 29497023
DOI: 10.1107/S2053230X18002819

実験手法

X-RAY DIFFRACTION (3.5 Å)