5NP6

70S structure prior to bypassing

これはPDB形式変換不可エントリーです。

5NP6 の概要

• エントリーDOI: 10.2210/pdb5np6/pdb
• EMDBエントリー: 3618
• 分子名称: mRNA, 30S ribosomal protein S7, 30S ribosomal protein S8, ... (57 entities in total)
• 機能のキーワード: ribosome, translation, bypassing, protein
• 由来する生物種: Escherichia coli (strain K12); 詳細
• タンパク質・核酸の鎖数: 57
• 化学式量合計: 2180309.45

構造登録者

Agirrezabala, X.,Samatova, E.,Klimova, M.,Zamora, M.,Gil-Carton, D.,Rodnina, M.,Valle, M. (登録日: 2017-04-13, 公開日: 2017-06-14, 最終更新日: 2024-04-24)

主引用文献

Agirrezabala, X.,Samatova, E.,Klimova, M.,Zamora, M.,Gil-Carton, D.,Rodnina, M.V.,Valle, M.
Ribosome rearrangements at the onset of translational bypassing.
Sci Adv, 3:e1700147-e1700147, 2017

PubMed Abstract: Bypassing is a recoding event that leads to the translation of two distal open reading frames into a single polypeptide chain. We present the structure of a translating ribosome stalled at the bypassing take-off site of of bacteriophage T4. The nascent peptide in the exit tunnel anchors the P-site peptidyl-tRNA to the ribosome and locks an inactive conformation of the peptidyl transferase center (PTC). The mRNA forms a short dynamic hairpin in the decoding site. The ribosomal subunits adopt a rolling conformation in which the rotation of the small subunit around its long axis causes the opening of the A-site region. Together, PTC conformation and mRNA structure safeguard against premature termination and read-through of the stop codon and reconfigure the ribosome to a state poised for take-off and sliding along the noncoding mRNA gap.

PubMed: 28630923
DOI: 10.1126/sciadv.1700147

実験手法

ELECTRON MICROSCOPY (3.6 Å)