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5NI1

CryoEM structure of haemoglobin at 3.2 A determined with the Volta phase plate

Replaces:  5ME2
Summary for 5NI1
Entry DOI10.2210/pdb5ni1/pdb
EMDB information3488
DescriptorHemoglobin subunit alpha, Hemoglobin subunit beta, PROTOPORPHYRIN IX CONTAINING FE, ... (4 entities in total)
Functional Keywordsvolta phase plate, single particle analysis, hemoglobin, oxygen transport
Biological sourceHomo sapiens (Human)
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Total number of polymer chains4
Total formula weight64547.05
Authors
Khoshouei, M.,Radjainia, M.,Bunker, R.,Baumeister, W.,Danev, R. (deposition date: 2017-03-22, release date: 2017-04-12, Last modification date: 2024-05-08)
Primary citationKhoshouei, M.,Radjainia, M.,Baumeister, W.,Danev, R.
Cryo-EM structure of haemoglobin at 3.2 angstrom determined with the Volta phase plate.
Nat Commun, 8:16099-16099, 2017
Cited by
PubMed Abstract: With the advent of direct electron detectors, the perspectives of cryo-electron microscopy (cryo-EM) have changed in a profound way. These cameras are superior to previous detectors in coping with the intrinsically low contrast and beam-induced motion of radiation-sensitive organic materials embedded in amorphous ice, and hence they have enabled the structure determination of many macromolecular assemblies to atomic or near-atomic resolution. Nevertheless, there are still limitations and one of them is the size of the target structure. Here, we report the use of a Volta phase plate in determining the structure of human haemoglobin (64 kDa) at 3.2 Å. Our results demonstrate that this method can be applied to complexes that are significantly smaller than those previously studied by conventional defocus-based approaches. Cryo-EM is now close to becoming a fast and cost-effective alternative to crystallography for high-resolution protein structure determination.
PubMed: 28665412
DOI: 10.1038/ncomms16099
PDB entries with the same primary citation
Experimental method
ELECTRON MICROSCOPY (3.2 Å)
Structure validation

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