5NGN
Lybatide 2, a cystine-rich peptide from Lycium barbarum
Summary for 5NGN
| Entry DOI | 10.2210/pdb5ngn/pdb |
| Descriptor | lybatide 2, GLYCEROL, ACETONITRILE, ... (5 entities in total) |
| Functional Keywords | cysteine-rich peptide, pi-helix, wolfberry, disulfide bonds, plant protein |
| Biological source | Lycium barbarum (goji berry) |
| Total number of polymer chains | 3 |
| Total formula weight | 11334.63 |
| Authors | Lei, J.,Tan, W.L.,Sakai, N.,Hilgenfeld, R. (deposition date: 2017-03-18, release date: 2017-07-26, Last modification date: 2024-10-16) |
| Primary citation | Tan, W.L.,Wong, K.H.,Lei, J.,Sakai, N.,Tan, H.W.,Hilgenfeld, R.,Tam, J.P. Lybatides from Lycium barbarum Contain An Unusual Cystine-stapled Helical Peptide Scaffold. Sci Rep, 7:5194-5194, 2017 Cited by PubMed Abstract: Cysteine-rich peptides (CRPs) of 2-6 kDa are generally thermally and proteolytically stable because of their multiple cross-bracing disulfide bonds. Here, we report the discovery and characterization of two novel cystine-stapled CRPs, designated lybatide 1 and 2 (lyba1 and lyba2), from the cortex of Lycium barbarum root. Lybatides, 32 to 33 amino acids in length, are hyperstable and display a novel disulfide connectivity with a cysteine motif of C-C-C-C-CC-CC which contains two pairs of adjacent cysteines (-CC-CC). X-ray structure analysis revealed the presence of a single cystine-stabilized (α + π)-helix in lyba2, a rare feature of CRPs. Together, our results suggest that lybatides, one of the smallest four-disulfide-constrained plant CRPs, is a new family of CRPs. Additionally, this study provides new insights into the molecular diversity of plant cysteine-rich peptides and the unusual lybatide scaffold could be developed as a useful template for peptide engineering and therapeutic development. PubMed: 28701689DOI: 10.1038/s41598-017-05037-1 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.48 Å) |
Structure validation
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