5NEJ

CryoEM Structure of Foot and Mouth Disease Virus O1 Manisa

Summary for 5NEJ

• Entry DOI: 10.2210/pdb5nej/pdb
• EMDB information: 3631
• Descriptor: O1 Manisa VP1, O1 Manisa VP2, O1 Manisa VP3, ... (4 entities in total)
• Functional Keywords: foot and mouth disease virus, fmdv, virus, opanasia
• Biological source: Foot-and-mouth disease virus; More
• Cellular location: Host cytoplasmic vesicle membrane ; Peripheral membrane protein ; Cytoplasmic side . Virion : Q6PMW3 Q6PMW3 Q80B23 E1ACS1
• Total number of polymer chains: 4
• Total formula weight: 80339.81

Authors

Kotecha, A.,Stuart, D. (deposition date: 2017-03-10, release date: 2017-06-21, Last modification date: 2024-05-15)

Primary citation

Kotecha, A.,Wang, Q.,Dong, X.,Ilca, S.L.,Ondiviela, M.,Zihe, R.,Seago, J.,Charleston, B.,Fry, E.E.,Abrescia, N.G.A.,Springer, T.A.,Huiskonen, J.T.,Stuart, D.I.
Rules of engagement between alpha v beta 6 integrin and foot-and-mouth disease virus.
Nat Commun, 8:15408-15408, 2017

PubMed Abstract: Foot-and-mouth disease virus (FMDV) mediates cell entry by attachment to an integrin receptor, generally αvβ6, via a conserved arginine-glycine-aspartic acid (RGD) motif in the exposed, antigenic, GH loop of capsid protein VP1. Infection can also occur in tissue culture adapted virus in the absence of integrin via acquired basic mutations interacting with heparin sulphate (HS); this virus is attenuated in natural infections. HS interaction has been visualized at a conserved site in two serotypes suggesting a propensity for sulfated-sugar binding. Here we determined the interaction between αvβ6 and two tissue culture adapted FMDV strains by cryo-electron microscopy. In the preferred mode of engagement, the fully open form of the integrin, hitherto unseen at high resolution, attaches to an extended GH loop via interactions with the RGD motif plus downstream hydrophobic residues. In addition, an N-linked sugar of the integrin attaches to the previously identified HS binding site, suggesting a functional role.

PubMed: 28534487
DOI: 10.1038/ncomms15408

Experimental method

ELECTRON MICROSCOPY (3.1 Å)