5N9Q
Structure of A. thaliana RCD1(468-567)
Summary for 5N9Q
| Entry DOI | 10.2210/pdb5n9q/pdb |
| NMR Information | BMRB: 27034 |
| Descriptor | Inactive poly [ADP-ribose] polymerase RCD1 (1 entity in total) |
| Functional Keywords | helical bundle, transferase |
| Biological source | Arabidopsis thaliana (Thale cress) |
| Total number of polymer chains | 1 |
| Total formula weight | 13935.75 |
| Authors | Tossavainen, H.,Hellman, M.,Vainonen, J.,Kangasjarvi, J.,Permi, P. (deposition date: 2017-02-27, release date: 2018-03-14, Last modification date: 2024-06-19) |
| Primary citation | Shapiguzov, A.,Vainonen, J.P.,Hunter, K.,Tossavainen, H.,Tiwari, A.,Jarvi, S.,Hellman, M.,Aarabi, F.,Alseekh, S.,Wybouw, B.,Van Der Kelen, K.,Nikkanen, L.,Krasensky-Wrzaczek, J.,Sipari, N.,Keinanen, M.,Tyystjarvi, E.,Rintamaki, E.,De Rybel, B.,Salojarvi, J.,Van Breusegem, F.,Fernie, A.R.,Brosche, M.,Permi, P.,Aro, E.M.,Wrzaczek, M.,Kangasjarvi, J. Arabidopsis RCD1 coordinates chloroplast and mitochondrial functions through interaction with ANAC transcription factors. Elife, 8:-, 2019 Cited by PubMed Abstract: Reactive oxygen species (ROS)-dependent signaling pathways from chloroplasts and mitochondria merge at the nuclear protein RADICAL-INDUCED CELL DEATH1 (RCD1). RCD1 interacts in vivo and suppresses the activity of the transcription factors ANAC013 and ANAC017, which mediate a ROS-related retrograde signal originating from mitochondrial complex III. Inactivation of leads to increased expression of mitochondrial dysfunction stimulon (MDS) genes regulated by ANAC013 and ANAC017. Accumulating MDS gene products, including alternative oxidases (AOXs), affect redox status of the chloroplasts, leading to changes in chloroplast ROS processing and increased protection of photosynthetic apparatus. ROS alter the abundance, thiol redox state and oligomerization of the RCD1 protein in vivo, providing feedback control on its function. RCD1-dependent regulation is linked to chloroplast signaling by 3'-phosphoadenosine 5'-phosphate (PAP). Thus, RCD1 integrates organellar signaling from chloroplasts and mitochondria to establish transcriptional control over the metabolic processes in both organelles. PubMed: 30767893DOI: 10.7554/eLife.43284 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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