5N74

Microtubule end binding protein complex

Summary for 5N74

• Entry DOI: 10.2210/pdb5n74/pdb
• Descriptor: Microtubule-associated protein RP/EB family member 1, Karyogamy protein KAR9 (3 entities in total)
• Functional Keywords: microtubule end binding protein complex, microtubule binding protein
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 16
• Total formula weight: 73699.69

Authors

Kumar, A.,Steinmetz, M. (deposition date: 2017-02-18, release date: 2017-06-14, Last modification date: 2024-01-17)

Primary citation

Kumar, A.,Manatschal, C.,Rai, A.,Grigoriev, I.,Degen, M.S.,Jaussi, R.,Kretzschmar, I.,Prota, A.E.,Volkmer, R.,Kammerer, R.A.,Akhmanova, A.,Steinmetz, M.O.
Short Linear Sequence Motif LxxPTPh Targets Diverse Proteins to Growing Microtubule Ends.
Structure, 25:924-932.e4, 2017

PubMed Abstract: Microtubule plus-end tracking proteins (+TIPs) are involved in virtually all microtubule-based processes. End-binding (EB) proteins are considered master regulators of +TIP interaction networks, since they autonomously track growing microtubule ends and recruit a plethora of proteins to this location. Two major EB-interacting elements have been described: CAP-Gly domains and linear SxIP sequence motifs. Here, we identified LxxPTPh as a third EB-binding motif that enables major +TIPs to interact with EBs at microtubule ends. In contrast to EB-SxIP and EB-CAP-Gly, the EB-LxxPTPh binding mode does not depend on the C-terminal tail region of EB. Our study reveals that +TIPs developed additional strategies besides CAP-Gly and SxIP to target EBs at growing microtubule ends. They further provide a unique basis to discover novel +TIPs, and to dissect the role of key interaction nodes and their differential regulation for hierarchical +TIP network organization and function in eukaryotic organisms.

PubMed: 28552577
DOI: 10.1016/j.str.2017.04.010

Experimental method

X-RAY DIFFRACTION (2.3 Å)