5N5Y
Cryo-EM structure of RNA polymerase I in complex with Rrn3 and Core Factor (Orientation III)
Summary for 5N5Y
| Entry DOI | 10.2210/pdb5n5y/pdb |
| EMDB information | 3590 |
| Descriptor | DNA-directed RNA polymerase I subunit RPA190, DNA-directed RNA polymerases I, II, and III subunit RPABC5, DNA-directed RNA polymerases I and III subunit RPAC2, ... (21 entities in total) |
| Functional Keywords | transcription, rna polymerase i initiation |
| Biological source | Saccharomyces cerevisiae (Baker's yeast) More |
| Total number of polymer chains | 18 |
| Total formula weight | 885697.37 |
| Authors | Engel, C.,Gubbey, T.,Neyer, S.,Sainsbury, S.,Oberthuer, C.,Baejen, C.,Bernecky, C.,Cramer, P. (deposition date: 2017-02-14, release date: 2017-04-05, Last modification date: 2018-10-03) |
| Primary citation | Engel, C.,Gubbey, T.,Neyer, S.,Sainsbury, S.,Oberthuer, C.,Baejen, C.,Bernecky, C.,Cramer, P. Structural Basis of RNA Polymerase I Transcription Initiation. Cell, 169:120-131.e22, 2017 Cited by PubMed Abstract: Transcription initiation at the ribosomal RNA promoter requires RNA polymerase (Pol) I and the initiation factors Rrn3 and core factor (CF). Here, we combine X-ray crystallography and cryo-electron microscopy (cryo-EM) to obtain a molecular model for basal Pol I initiation. The three-subunit CF binds upstream promoter DNA, docks to the Pol I-Rrn3 complex, and loads DNA into the expanded active center cleft of the polymerase. DNA unwinding between the Pol I protrusion and clamp domains enables cleft contraction, resulting in an active Pol I conformation and RNA synthesis. Comparison with the Pol II system suggests that promoter specificity relies on a distinct "bendability" and "meltability" of the promoter sequence that enables contacts between initiation factors, DNA, and polymerase. PubMed: 28340337DOI: 10.1016/j.cell.2017.03.003 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (7.7 Å) |
Structure validation
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