5N11

Crystal structure of Human beta1-coronavirus OC43 NL/A/2005 Hemagglutinin-Esterase

5N11 の概要

• エントリーDOI: 10.2210/pdb5n11/pdb
• 分子名称: Hemagglutinin-esterase, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (9 entities in total)
• 機能のキーワード: hemagglutin, esterase, hepatitis virus, coronavirus, viral protein, sialic acid
• 由来する生物種: Human coronavirus OC43 (HCoV-OC43)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 92969.91

構造登録者

Bakkers, M.J.G.,Feitsma, L.J.,de Groot, R.J.,Huizinga, E.G. (登録日: 2017-02-04, 公開日: 2017-03-22, 最終更新日: 2024-01-17)

主引用文献

Bakkers, M.J.,Lang, Y.,Feitsma, L.J.,Hulswit, R.J.,de Poot, S.A.,van Vliet, A.L.,Margine, I.,de Groot-Mijnes, J.D.,van Kuppeveld, F.J.,Langereis, M.A.,Huizinga, E.G.,de Groot, R.J.
Betacoronavirus Adaptation to Humans Involved Progressive Loss of Hemagglutinin-Esterase Lectin Activity.
Cell Host Microbe, 21:356-366, 2017

PubMed Abstract: Human beta1-coronavirus (β1CoV) OC43 emerged relatively recently through a single zoonotic introduction. Like related animal β1CoVs, OC43 uses 9-O-acetylated sialic acid as receptor determinant. β1CoV receptor binding is typically controlled by attachment/fusion spike protein S and receptor-binding/receptor-destroying hemagglutinin-esterase protein HE. We show that following OC43's introduction into humans, HE-mediated receptor binding was selected against and ultimately lost through progressive accumulation of mutations in the HE lectin domain. Consequently, virion-associated receptor-destroying activity toward multivalent glycoconjugates was reduced and altered such that some clustered receptor populations are no longer cleaved. Loss of HE lectin function was also observed for another respiratory human coronavirus, HKU1. This thus appears to be an adaptation to the sialoglycome of the human respiratory tract and for replication in human airways. The findings suggest that the dynamics of virion-glycan interactions contribute to host tropism. Our observations are relevant also to other human respiratory viruses of zoonotic origin, particularly influenza A virus.

PubMed: 28279346
DOI: 10.1016/j.chom.2017.02.008

実験手法

X-RAY DIFFRACTION (2.45 Å)