5N07
Structure of the [4Fe-4S] form of the NO response regulator NsrR
Summary for 5N07
| Entry DOI | 10.2210/pdb5n07/pdb |
| Descriptor | HTH-type transcriptional repressor NsrR, IRON/SULFUR CLUSTER, SULFATE ION, ... (5 entities in total) |
| Functional Keywords | no sensor, iron sulfur cluster, transcription |
| Biological source | Streptomyces coelicolor A3(2) |
| Total number of polymer chains | 1 |
| Total formula weight | 17986.26 |
| Authors | Volbeda, A.,Fontecilla-Camps, J.C. (deposition date: 2017-02-02, release date: 2017-04-26, Last modification date: 2024-05-08) |
| Primary citation | Volbeda, A.,Dodd, E.L.,Darnault, C.,Crack, J.C.,Renoux, O.,Hutchings, M.I.,Le Brun, N.E.,Fontecilla-Camps, J.C. Crystal structures of the NO sensor NsrR reveal how its iron-sulfur cluster modulates DNA binding. Nat Commun, 8:15052-15052, 2017 Cited by PubMed Abstract: NsrR from Streptomyces coelicolor (Sc) regulates the expression of three genes through the progressive degradation of its [4Fe-4S] cluster on nitric oxide (NO) exposure. We report the 1.95 Å resolution crystal structure of dimeric holo-ScNsrR and show that the cluster is coordinated by the three invariant Cys residues from one monomer and, unexpectedly, Asp8 from the other. A cavity map suggests that NO displaces Asp8 as a cluster ligand and, while D8A and D8C variants remain NO sensitive, DNA binding is affected. A structural comparison of holo-ScNsrR with an apo-IscR-DNA complex shows that the [4Fe-4S] cluster stabilizes a turn between ScNsrR Cys93 and Cys99 properly oriented to interact with the DNA backbone. In addition, an apo ScNsrR structure suggests that Asn97 from this turn, along with Arg12, which forms a salt-bridge with Asp8, are instrumental in modulating the position of the DNA recognition helix region relative to its major groove. PubMed: 28425466DOI: 10.1038/ncomms15052 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.95 Å) |
Structure validation
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