5MY1
E. coli expressome
Summary for 5MY1
| Entry DOI | 10.2210/pdb5my1/pdb |
| EMDB information | 3580 |
| Descriptor | 16S ribosomal RNA, 30S ribosomal protein S11, 30S ribosomal protein S12, ... (26 entities in total) |
| Functional Keywords | expressome ribosome rna polymerase, ribosome |
| Biological source | Escherichia coli K-12 More |
| Total number of polymer chains | 26 |
| Total formula weight | 1177189.17 |
| Authors | Kohler, R.,Mooney, R.A.,Mills, D.J.,Kostrewa, D.,Landick, R.,Cramer, P. (deposition date: 2017-01-25, release date: 2017-04-26, Last modification date: 2024-05-15) |
| Primary citation | Kohler, R.,Mooney, R.A.,Mills, D.J.,Landick, R.,Cramer, P. Architecture of a transcribing-translating expressome. Science, 356:194-197, 2017 Cited by PubMed Abstract: DNA transcription is functionally coupled to messenger RNA (mRNA) translation in bacteria, but how this is achieved remains unclear. Here we show that RNA polymerase (RNAP) and the ribosome of can form a defined transcribing and translating "expressome" complex. The cryo-electron microscopic structure of the expressome reveals continuous protection of ~30 nucleotides of mRNA extending from the RNAP active center to the ribosome decoding center. The RNAP-ribosome interface includes the RNAP subunit α carboxyl-terminal domain, which is required for RNAP-ribosome interaction in vitro and for pronounced cell growth defects upon translation inhibition in vivo, consistent with its function in transcription-translation coupling. The expressome structure can only form during transcription elongation and explains how translation can prevent transcriptional pausing, backtracking, and termination. PubMed: 28408604DOI: 10.1126/science.aal3059 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (7.6 Å) |
Structure validation
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