5MTA
G-quadruplex formed within promoters of Plasmodium falciparum B var genes
Summary for 5MTA
| Entry DOI | 10.2210/pdb5mta/pdb |
| NMR Information | BMRB: 34083 |
| Descriptor | UpsB-Q-1, DNA (34-MER) (1 entity in total) |
| Functional Keywords | g-quadruplex, nucleic acid, long loop, nmr spectroscopy, malaria, dna |
| Biological source | Plasmodium falciparum (malaria parasite P. falciparum) |
| Total number of polymer chains | 1 |
| Total formula weight | 10664.85 |
| Authors | Juribasic Kulcsar, M.,Plavec, J. (deposition date: 2017-01-09, release date: 2018-02-28, Last modification date: 2025-07-30) |
| Primary citation | Juribasic Kulcsar, M.,Gabelica, V.,Plavec, J. Solution-State Structure of a Long-Loop G-Quadruplex Formed Within Promoters of Plasmodium falciparum B var Genes. Chemistry, 30:e202401190-e202401190, 2024 Cited by PubMed Abstract: We report the high-resolution NMR solution-state structure of an intramolecular G-quadruplex with a diagonal loop of ten nucleotides. The G-quadruplex is formed by a 34-nt DNA sequence, d[CAGTAGTATACTAGTAGT], named UpsB-Q-1. This sequence is found within promoters of the var genes of Plasmodium falciparum, which play a key role in malaria pathogenesis and evasion of the immune system. The [3+1]-hybrid G-quadruplex formed under physiologically relevant conditions exhibits a unique equilibrium between two structures, both stabilized by base stacking and non-canonical hydrogen bonding. Unique equilibrium of the two closely related 3D structures originates from a North-South repuckering of deoxyribose moiety of residue T27 in the lateral loop. Besides the 12 guanines involved in three G-quartets, most residues in loop regions are involved in interactions at both G-quartet-loop interfaces. PubMed: 38647110DOI: 10.1002/chem.202401190 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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