5MSM

Structure of the Dcc1-Ctf8-Ctf18C Trimer

Summary for 5MSM

• Entry DOI: 10.2210/pdb5msm/pdb
• Descriptor: Sister chromatid cohesion protein DCC1, Chromosome transmission fidelity protein 8, Chromosome transmission fidelity protein 18, ... (4 entities in total)
• Functional Keywords: winged-helix, dna repair, cell cycle
• Biological source: Saccharomyces cerevisiae S288c; More
• Cellular location: Nucleus : P38877 P49956
• Total number of polymer chains: 6
• Total formula weight: 136478.40

Authors

Wade, B.O.,Singleton, M.R. (deposition date: 2017-01-05, release date: 2017-02-15, Last modification date: 2024-01-17)

Primary citation

Wade, B.O.,Liu, H.W.,Samora, C.P.,Uhlmann, F.,Singleton, M.R.
Structural studies of RFC(C)(tf18) reveal a novel chromatin recruitment role for Dcc1.
EMBO Rep., 18:558-568, 2017

PubMed Abstract: Replication factor C complexes load and unload processivity clamps from DNA and are involved in multiple DNA replication and repair pathways. The RFC variant complex is required for activation of the intra-S-phase checkpoint at stalled replication forks and aids the establishment of sister chromatid cohesion. Unlike other RFC complexes, RFC contains two non-Rfc subunits, Dcc1 and Ctf8. Here, we present the crystal structure of the Dcc1-Ctf8 heterodimer bound to the C-terminus of Ctf18. We find that the C-terminus of Dcc1 contains three-winged helix domains, which bind to both ssDNA and dsDNA We further show that these domains are required for full recruitment of the complex to chromatin, and correct activation of the replication checkpoint. These findings provide the first structural data on a eukaryotic seven-subunit clamp loader and define a new biochemical activity for Dcc1.

PubMed: 28188145
DOI: 10.15252/embr.201642825

Experimental method

X-RAY DIFFRACTION (2.29 Å)