5MP7

Crystal structure of phosphoribosylpyrophosphate synthetase from Mycobacterium smegmatis

5MP7 の概要

• エントリーDOI: 10.2210/pdb5mp7/pdb
• 分子名称: Ribose-phosphate pyrophosphokinase, ACETATE ION (3 entities in total)
• 機能のキーワード: phosphoribosylpyrophosphate synthetase, prpp synthetase, mycobacterium smegmatis, transferase
• 由来する生物種: Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 106987.82

構造登録者

Donini, S.,Garavaglia, S.,Ferraris, D.M.,Miggiano, R.,Mori, S.,Shibayama, K.,Rizzi, M. (登録日: 2016-12-16, 公開日: 2017-04-26, 最終更新日: 2024-01-17)

主引用文献

Donini, S.,Garavaglia, S.,Ferraris, D.M.,Miggiano, R.,Mori, S.,Shibayama, K.,Rizzi, M.
Biochemical and structural investigations on phosphoribosylpyrophosphate synthetase from Mycobacterium smegmatis.
PLoS ONE, 12:e0175815-e0175815, 2017

PubMed Abstract: Mycobacterium smegmatis represents one model for studying the biology of its pathogenic relative Mycobacterium tuberculosis. The structural characterization of a M. tuberculosis ortholog protein can serve as a valid tool for the development of molecules active against the M. tuberculosis target. In this context, we report the biochemical and structural characterization of M. smegmatis phosphoribosylpyrophosphate synthetase (PrsA), the ortholog of M. tuberculosis PrsA, the unique enzyme responsible for the synthesis of phosphoribosylpyrophosphate (PRPP). PRPP is a key metabolite involved in several biosynthetic pathways including those for histidine, tryptophan, nucleotides and decaprenylphosphoryl-arabinose, an essential precursor for the mycobacterial cell wall biosynthesis. Since M. tuberculosis PrsA has been validated as a drug target for the development of antitubercular agents, the data presented here will add to the knowledge of the mycobacterial enzyme and could contribute to the development of M. tuberculosis PrsA inhibitors of potential pharmacological interest.

PubMed: 28419153
DOI: 10.1371/journal.pone.0175815

実験手法

X-RAY DIFFRACTION (2.4 Å)