5MND
SFX structure of Cydia pomonella granulovirus using a double flow-focusing nozzle
Summary for 5MND
| Entry DOI | 10.2210/pdb5mnd/pdb |
| Descriptor | Granulin (2 entities in total) |
| Functional Keywords | sfx, granulovirus, in vivo crystals, nanocrystals, viral protein |
| Biological source | Cydia pomonella granulosis virus (isolate Mexico/1963) |
| Total number of polymer chains | 1 |
| Total formula weight | 28783.86 |
| Authors | Oberthuer, D.,Chapman, H.,Doerner, K.,Xavier, P.L. (deposition date: 2016-12-13, release date: 2017-03-29, Last modification date: 2024-10-16) |
| Primary citation | Oberthuer, D.,Knoska, J.,Wiedorn, M.O.,Beyerlein, K.R.,Bushnell, D.A.,Kovaleva, E.G.,Heymann, M.,Gumprecht, L.,Kirian, R.A.,Barty, A.,Mariani, V.,Tolstikova, A.,Adriano, L.,Awel, S.,Barthelmess, M.,Dorner, K.,Xavier, P.L.,Yefanov, O.,James, D.R.,Nelson, G.,Wang, D.,Calvey, G.,Chen, Y.,Schmidt, A.,Szczepek, M.,Frielingsdorf, S.,Lenz, O.,Snell, E.,Robinson, P.J.,Sarler, B.,Belsak, G.,Macek, M.,Wilde, F.,Aquila, A.,Boutet, S.,Liang, M.,Hunter, M.S.,Scheerer, P.,Lipscomb, J.D.,Weierstall, U.,Kornberg, R.D.,Spence, J.C.,Pollack, L.,Chapman, H.N.,Bajt, S. Double-flow focused liquid injector for efficient serial femtosecond crystallography. Sci Rep, 7:44628-44628, 2017 Cited by PubMed Abstract: Serial femtosecond crystallography requires reliable and efficient delivery of fresh crystals across the beam of an X-ray free-electron laser over the course of an experiment. We introduce a double-flow focusing nozzle to meet this challenge, with significantly reduced sample consumption, while improving jet stability over previous generations of nozzles. We demonstrate its use to determine the first room-temperature structure of RNA polymerase II at high resolution, revealing new structural details. Moreover, the double-flow focusing nozzles were successfully tested with three other protein samples and the first room temperature structure of an extradiol ring-cleaving dioxygenase was solved by utilizing the improved operation and characteristics of these devices [corrected]. PubMed: 28300169DOI: 10.1038/srep44628 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.56 Å) |
Structure validation
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