5MLU

Crystal structure of the PFV GAG CBS bound to a mononucleosome

5MLU の概要

• エントリーDOI: 10.2210/pdb5mlu/pdb
• 分子名称: Histone H3.2, Histone H4, Histone H2A type 1, ... (10 entities in total)
• 機能のキーワード: nucleosome, gag, prototype foamy virus (pfv), complex, protein, dna, dna binding protein
• 由来する生物種: Xenopus laevis (African clawed frog); 詳細
• 細胞内の位置: Nucleus: P84233 P62799 P06897
• タンパク質・核酸の鎖数: 11
• 化学式量合計: 177476.78

構造登録者

Pye, V.E.,Maskell, D.P.,Lesbats, P.,Cherepanov, P. (登録日: 2016-12-07, 公開日: 2017-05-10, 最終更新日: 2024-01-17)

主引用文献

Lesbats, P.,Serrao, E.,Maskell, D.P.,Pye, V.E.,O'Reilly, N.,Lindemann, D.,Engelman, A.N.,Cherepanov, P.
Structural basis for spumavirus GAG tethering to chromatin.
Proc. Natl. Acad. Sci. U.S.A., 114:5509-5514, 2017

PubMed Abstract: The interactions between a retrovirus and host cell chromatin that underlie integration and provirus expression are poorly understood. The prototype foamy virus (PFV) structural protein GAG associates with chromosomes via a chromatin-binding sequence (CBS) located within its C-terminal region. Here, we show that the PFV CBS is essential and sufficient for a direct interaction with nucleosomes and present a crystal structure of the CBS bound to a mononucleosome. The CBS interacts with the histone octamer, engaging the H2A-H2B acidic patch in a manner similar to other acidic patch-binding proteins such as herpesvirus latency-associated nuclear antigen (LANA). Substitutions of the invariant arginine anchor residue in GAG result in global redistribution of PFV and macaque simian foamy virus (SFV) integration sites toward centromeres, dampening the resulting proviral expression without affecting the overall efficiency of integration. Our findings underscore the importance of retroviral structural proteins for integration site selection and the avoidance of genomic junkyards.

PubMed: 28490494
DOI: 10.1073/pnas.1621159114

実験手法

X-RAY DIFFRACTION (2.8 Å)