5MLC

Cryo-EM structure of the spinach chloroplast ribosome reveals the location of plastid-specific ribosomal proteins and extensions

5MLC の概要

• エントリーDOI: 10.2210/pdb5mlc/pdb
• EMDBエントリー: 3525
• 分子名称: 23S ribosomal RNA, chloroplastic, 50S ribosomal protein L13, chloroplastic, 50S ribosomal protein L14, chloroplastic, ... (32 entities in total)
• 機能のキーワード: chloroplast, ribosome, ribosomal protein, translation
• 由来する生物種: Spinacia oleracea (Spinach); 詳細
• タンパク質・核酸の鎖数: 32
• 化学式量合計: 1573211.91

構造登録者

Graf, M.,Arenz, S.,Huter, P.,Doenhoefer, A.,Novacek, J.,Wilson, D.N. (登録日: 2016-12-06, 公開日: 2016-12-28, 最終更新日: 2024-10-16)

主引用文献

Graf, M.,Arenz, S.,Huter, P.,Donhofer, A.,Novacek, J.,Wilson, D.N.
Cryo-EM structure of the spinach chloroplast ribosome reveals the location of plastid-specific ribosomal proteins and extensions.
Nucleic Acids Res., 45:2887-2896, 2017

PubMed Abstract: Ribosomes are the protein synthesizing machines of the cell. Recent advances in cryo-EM have led to the determination of structures from a variety of species, including bacterial 70S and eukaryotic 80S ribosomes as well as mitoribosomes from eukaryotic mitochondria, however, to date high resolution structures of plastid 70S ribosomes have been lacking. Here we present a cryo-EM structure of the spinach chloroplast 70S ribosome, with an average resolution of 5.4 Å for the small 30S subunit and 3.6 Å for the large 50S ribosomal subunit. The structure reveals the location of the plastid-specific ribosomal proteins (RPs) PSRP1, PSRP4, PSRP5 and PSRP6 as well as the numerous plastid-specific extensions of the RPs. We discover many features by which the plastid-specific extensions stabilize the ribosome via establishing additional interactions with surrounding ribosomal RNA and RPs. Moreover, we identify a large conglomerate of plastid-specific protein mass adjacent to the tunnel exit site that could facilitate interaction of the chloroplast ribosome with the thylakoid membrane and the protein-targeting machinery. Comparing the Escherichia coli 70S ribosome with that of the spinach chloroplast ribosome provides detailed insight into the co-evolution of RP and rRNA.

PubMed: 27986857
DOI: 10.1093/nar/gkw1272

実験手法

ELECTRON MICROSCOPY (3.6 Å)