5MJU

Structure of the thermostabilized EAAT1 cryst mutant in complex with the competititve inhibitor TFB-TBOA and the allosteric inhibitor UCPH101

Summary for 5MJU

• Entry DOI: 10.2210/pdb5mju/pdb
• Descriptor: Excitatory amino acid transporter 1,Neutral amino acid transporter B(0),Excitatory amino acid transporter 1, 2-Amino-5,6,7,8-tetrahydro-4-(4-methoxyphenyl)-7-(naphthalen-1-yl)-5-oxo-4H-chromene-3-carbonitrile, (2~{S},3~{S})-2-azanyl-3-[[3-[[4-(trifluoromethyl)phenyl]carbonylamino]phenyl]methoxy]butanedioic acid (3 entities in total)
• Functional Keywords: excitatory aminoacid transporter 1, human glutamate transporter, tfb-tboa, ucph-101, transport protein
• Biological source: Homo sapiens (Human); More
• Cellular location: Membrane; Multi-pass membrane protein: P43003
• Total number of polymer chains: 1
• Total formula weight: 57361.20

Authors

Canul-Tec, J.,Assal, R.,Legrand, P.,Reyes, N. (deposition date: 2016-12-01, release date: 2017-04-19, Last modification date: 2024-01-17)

Primary citation

Canul-Tec, J.C.,Assal, R.,Cirri, E.,Legrand, P.,Brier, S.,Chamot-Rooke, J.,Reyes, N.
Structure and allosteric inhibition of excitatory amino acid transporter 1.
Nature, 544:446-451, 2017

PubMed Abstract: Human members of the solute carrier 1 (SLC1) family of transporters take up excitatory neurotransmitters in the brain and amino acids in peripheral organs. Dysregulation of the function of SLC1 transporters is associated with neurodegenerative disorders and cancer. Here we present crystal structures of a thermostabilized human SLC1 transporter, the excitatory amino acid transporter 1 (EAAT1), with and without allosteric and competitive inhibitors bound. The structures reveal architectural features of the human transporters, such as intra- and extracellular domains that have potential roles in transport function, regulation by lipids and post-translational modifications. The coordination of the allosteric inhibitor in the structures and the change in the transporter dynamics measured by hydrogen-deuterium exchange mass spectrometry reveal a mechanism of inhibition, in which the transporter is locked in the outward-facing states of the transport cycle. Our results provide insights into the molecular mechanisms underlying the function and pharmacology of human SLC1 transporters.

PubMed: 28424515
DOI: 10.1038/nature22064

Experimental method

X-RAY DIFFRACTION (3.71 Å)