5MHD

Biosynthetic engineered A22S-B3K-B31R human insulin monomer structure in water/acetonitrile solutions.

Summary for 5MHD

• Entry DOI: 10.2210/pdb5mhd/pdb
• NMR Information: BMRB: 34070
• Descriptor: Insulin (2 entities in total)
• Functional Keywords: human insulin, water/acetonitrile solution, mutant, hormone
• Biological source: Homo sapiens (Human); More
• Total number of polymer chains: 2
• Total formula weight: 6077.00

Authors

Bocian, W.,Kozerski, L.,Bednarek, E.,Sitkowski, J. (deposition date: 2016-11-24, release date: 2017-08-09, Last modification date: 2024-11-13)

Primary citation

Bednarek, E.,Sitkowski, J.,Bocian, W.,Borowicz, P.,Pucienniczak, G.,Stadnik, D.,Surmacz-Chwedoruk, W.,Jaworska, B.,Kozerski, L.
Structure and pharmaceutical formulation development of a new long-acting recombinant human insulin analog studied by NMR and MS.
J Pharm Biomed Anal, 135:126-132, 2017

PubMed Abstract: A monomer structure of a novel human insulin analog A22-B3-B31 (SK3R) has been characterized by NMR in water/acetonitrile solution and compared with the structure of human insulin (HIS) established in the same medium. The composition of the oligomer ensemble for neat insulins in water was qualitatively assessed by monitoring, derived from NMR experiment, translational diffusion coefficient Dix10ms, whose value is a population averaged of individual coefficients for species in oligomeric ensemble. Nanospray ESI/MS experiment was used to establish the masses of oligomers in pharmaceutical formulation of the SK3R insulin. The pharmacodynamic data were established and compared to insulin glargine characterized by the same profile of action in diabetics. The oligomerization process of insulin during development of pharmaceutical formulation with routinely used excipients has been studied using translation diffusion coefficient Dix10ms established in water solution. These properties were compared with those of human insulin (HIS) which is a standard reference for novel recombinant insulins.

PubMed: 28024260
DOI: 10.1016/j.jpba.2016.12.005

Experimental method

SOLUTION NMR