5ME3

Structure of the Scc2 C-terminus

5ME3 の概要

• エントリーDOI: 10.2210/pdb5me3/pdb
• 分子名称: Sister chromatid cohesion protein 2, unassigned sequence of Scc2, Scc2 unassigned sequence, ... (4 entities in total)
• 機能のキーワード: cohesin loader, heat repeat, scc2, cell cycle
• 由来する生物種: Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) (Yeast); 詳細
• 細胞内の位置: Nucleus : Q750S2
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 268810.67

構造登録者

Chao, W.C.H.,Singleton, M.R. (登録日: 2016-11-14, 公開日: 2017-01-11, 最終更新日: 2024-05-08)

主引用文献

Chao, W.C.,Murayama, Y.,Munoz, S.,Jones, A.W.,Wade, B.O.,Purkiss, A.G.,Hu, X.W.,Borg, A.,Snijders, A.P.,Uhlmann, F.,Singleton, M.R.
Structure of the cohesin loader Scc2.
Nat Commun, 8:13952-13952, 2017

PubMed Abstract: The functions of cohesin are central to genome integrity, chromosome organization and transcription regulation through its prevention of premature sister-chromatid separation and the formation of DNA loops. The loading of cohesin onto chromatin depends on the Scc2-Scc4 complex; however, little is known about how it stimulates the cohesion-loading activity. Here we determine the large 'hook' structure of Scc2 responsible for catalysing cohesin loading. We identify key Scc2 surfaces that are crucial for cohesin loading in vivo. With the aid of previously determined structures and homology modelling, we derive a pseudo-atomic structure of the full-length Scc2-Scc4 complex. Finally, using recombinantly purified Scc2-Scc4 and cohesin, we performed crosslinking mass spectrometry and interaction assays that suggest Scc2-Scc4 uses its modular structure to make multiple contacts with cohesin.

PubMed: 28059076
DOI: 10.1038/ncomms13952

実験手法

X-RAY DIFFRACTION (2.85 Å)