5MDY

Structure of ArfA and TtRF2 bound to the 70S ribosome (pre-accommodated state)

This is a large structure.

Summary for 5MDY

EMDB information3492
Descriptor23S ribosomal RNA, 16S ribosomal RNA, 5S ribosomal RNA, ... (62 entities in total)
Functional Keywordsribosome, arfa, rf2, trans-translation, 70s, 50s, 30s, rescue termination, cryo-em
Biological sourceEscherichia coli K-12
More
Total number of polymer chains58
Total molecular weight2262715.7
Authors
James, N.R.,Brown, A.,Gordiyenko, Y.,Ramakrishnan, V. (deposition date: 2016-11-13, release date: 2016-12-21, Last modification date: 2018-10-17)
Primary citation
James, N.R.,Brown, A.,Gordiyenko, Y.,Ramakrishnan, V.
Translational termination without a stop codon.
Science, 354:1437-1440, 2016
PubMed: 27934701 (PDB entries with the same primary citation)
DOI: 10.1126/science.aai9127
MImport into Mendeley
Experimental method
ELECTRON MICROSCOPY (3.35 Å)
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Structure validation

ClashscoreRamachandran outliersSidechain outliersRNA backbone4 0.5% 9.0% 0.46MetricValuePercentile RanksWorseBetterPercentile relative to all structuresPercentile relative to all EM structures

More Asymmetric unit images

Molmil generated image of 5mdy
no rotation
Molmil generated image of 5mdy
rotated about x axis by 90°
Molmil generated image of 5mdy
rotated about y axis by 90°
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