5M3M

Free monomeric RNA polymerase I at 4.0A resolution

Summary for 5M3M

EMDB informationEMD-4148
DescriptorDNA-directed RNA polymerase I subunit RPA190, DNA-directed RNA polymerase I subunit RPA135, DNA-directed RNA polymerases I and III subunit RPAC1, ... (16 entities in total)
Functional Keywordsrna polymerase i, transcription
Biological sourceSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Cellular locationNucleus, nucleolus  P10964 P22138 P07703 P32529 P22139 P28000 P40422 Q01080 P47006 P50106 P46669
Nucleus P20434 P20436
Cytoplasm  P20435
Total number of polymer chains14
Total molecular weight590832.46
Authors
Neyer, S.,Kunz, M.,Geiss, C.,Hantsche, M.,Hodirnau, V.-V.,Seybert, A.,Engel, C.,Scheffer, M.P.,Cramer, P.,Frangakis, A.S. (deposition date: 2016-10-15, release date: 2016-11-23, Last modification date: 2017-08-30)
Primary citation
Neyer, S.,Kunz, M.,Geiss, C.,Hantsche, M.,Hodirnau, V.V.,Seybert, A.,Engel, C.,Scheffer, M.P.,Cramer, P.,Frangakis, A.S.
Structure of RNA polymerase I transcribing ribosomal DNA genes.
Nature, 540:607-610, 2016
PubMed: 27842382 (PDB entries with the same primary citation)
DOI: 10.1038/nature20561
MImport into Mendeley
Experimental method
ELECTRON MICROSCOPY
?

Structure validation

ClashscoreRamachandran outliersSidechain outliers240.7%1.0%MetricValuePercentile RanksWorseBetterPercentile relative to all structuresPercentile relative to all EM structures

More Asymmetric unit images

Molmil generated image of 5m3m
no rotation
Molmil generated image of 5m3m
rotated about x axis by 90°
Molmil generated image of 5m3m
rotated about y axis by 90°
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