5M1J

Nonstop ribosomal complex bound with Dom34 and Hbs1

これはPDB形式変換不可エントリーです。

5M1J の概要

• エントリーDOI: 10.2210/pdb5m1j/pdb
• EMDBエントリー: 4140
• 分子名称: Protein DOM34, 40S ribosomal protein S4-A, 40S ribosomal protein S30-A, ... (87 entities in total)
• 機能のキーワード: mrna surveillance, ribosome
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast); 詳細
• タンパク質・核酸の鎖数: 82
• 化学式量合計: 3195268.10

構造登録者

Hilal, T.,Yamamoto, H.,Loerke, J.,Buerger, J.,Mielke, T.,Spahn, C.M.T. (登録日: 2016-10-07, 公開日: 2017-01-18, 最終更新日: 2024-05-15)

主引用文献

Hilal, T.,Yamamoto, H.,Loerke, J.,Burger, J.,Mielke, T.,Spahn, C.M.
Structural insights into ribosomal rescue by Dom34 and Hbs1 at near-atomic resolution.
Nat Commun, 7:13521-13521, 2016

PubMed Abstract: The surveillance of mRNA translation is imperative for homeostasis. Monitoring the integrity of the message is essential, as the translation of aberrant mRNAs leads to stalling of the translational machinery. During ribosomal rescue, arrested ribosomes are specifically recognized by the conserved eukaryotic proteins Dom34 and Hbs1, to initiate their recycling. Here we solve the structure of Dom34 and Hbs1 bound to a yeast ribosome programmed with a nonstop mRNA at 3.3 Å resolution using cryo-electron microscopy. The structure shows that Domain N of Dom34 is inserted into the upstream mRNA-binding groove via direct stacking interactions with conserved nucleotides of 18S rRNA. It senses the absence of mRNA at the A-site and part of the mRNA entry channel by direct competition. Thus, our analysis establishes the structural foundation for the recognition of aberrantly stalled 80S ribosomes by the Dom34·Hbs1·GTP complex during Dom34-mediated mRNA surveillance pathways.

PubMed: 27995908
DOI: 10.1038/ncomms13521

実験手法

ELECTRON MICROSCOPY (3.3 Å)