5LTY

Homeobox transcription factor CDX2 bound to methylated DNA

5LTY の概要

• エントリーDOI: 10.2210/pdb5lty/pdb
• 分子名称: DNA (5'-D(P*TP*TP*GP*TP*GP*TP*TP*TP*TP*AP*(5CM)P*GP*AP*CP*CP*TP*CP*C)-3'), Homeobox protein CDX-2, DNA (5'-D(P*GP*GP*AP*GP*GP*TP*(5CM)P*GP*TP*AP*AP*AP*AP*CP*AP*CP*AP*A)-3'), ... (4 entities in total)
• 機能のキーワード: transcription, transcription factor, methylated dna
• 由来する生物種: Homo sapiens (Human); 詳細
• 細胞内の位置: Nucleus: Q99626
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 39856.92

構造登録者

Morgunova, E.,Popov, A.,Taipale, J. (登録日: 2016-09-07, 公開日: 2017-05-31, 最終更新日: 2024-01-17)

主引用文献

Yin, Y.,Morgunova, E.,Jolma, A.,Kaasinen, E.,Sahu, B.,Khund-Sayeed, S.,Das, P.K.,Kivioja, T.,Dave, K.,Zhong, F.,Nitta, K.R.,Taipale, M.,Popov, A.,Ginno, P.A.,Domcke, S.,Yan, J.,Schubeler, D.,Vinson, C.,Taipale, J.
Impact of cytosine methylation on DNA binding specificities of human transcription factors.
Science, 356:-, 2017

PubMed Abstract: The majority of CpG dinucleotides in the human genome are methylated at cytosine bases. However, active gene regulatory elements are generally hypomethylated relative to their flanking regions, and the binding of some transcription factors (TFs) is diminished by methylation of their target sequences. By analysis of 542 human TFs with methylation-sensitive SELEX (systematic evolution of ligands by exponential enrichment), we found that there are also many TFs that prefer CpG-methylated sequences. Most of these are in the extended homeodomain family. Structural analysis showed that homeodomain specificity for methylcytosine depends on direct hydrophobic interactions with the methylcytosine 5-methyl group. This study provides a systematic examination of the effect of an epigenetic DNA modification on human TF binding specificity and reveals that many developmentally important proteins display preference for mCpG-containing sequences.

PubMed: 28473536
DOI: 10.1126/science.aaj2239

実験手法

X-RAY DIFFRACTION (2.66 Å)