5LTW

Complex of human 14-3-3 sigma CLU1 mutant with phosphorylated heat shock protein B6

5LTW の概要

• エントリーDOI: 10.2210/pdb5ltw/pdb
• 分子名称: 14-3-3 protein sigma, Heat shock protein beta-6, 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, ... (4 entities in total)
• 機能のキーワード: protein-protein complex, intrinsically disordered protein region(s), protein binding
• 由来する生物種: Homo sapiens (Human); 詳細
• 細胞内の位置: Cytoplasm: P31947 O14558
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 255926.60

構造登録者

Sluchanko, N.N.,Beelen, S.,Kulikova, A.A.,Weeks, S.D.,Antson, A.A.,Gusev, N.B.,Strelkov, S.V. (登録日: 2016-09-07, 公開日: 2017-02-01, 最終更新日: 2024-10-23)

主引用文献

Sluchanko, N.N.,Beelen, S.,Kulikova, A.A.,Weeks, S.D.,Antson, A.A.,Gusev, N.B.,Strelkov, S.V.
Structural Basis for the Interaction of a Human Small Heat Shock Protein with the 14-3-3 Universal Signaling Regulator.
Structure, 25:305-316, 2017

PubMed Abstract: By interacting with hundreds of protein partners, 14-3-3 proteins coordinate vital cellular processes. Phosphorylation of the small heat shock protein, HSPB6, within its intrinsically disordered N-terminal domain activates its interaction with 14-3-3, ultimately triggering smooth muscle relaxation. After analyzing the binding of an HSPB6-derived phosphopeptide to 14-3-3 using isothermal calorimetry and X-ray crystallography, we have determined the crystal structure of the complete assembly consisting of the 14-3-3 dimer and full-length HSPB6 dimer and further characterized this complex in solution using fluorescence spectroscopy, small-angle X-ray scattering, and limited proteolysis. We show that selected intrinsically disordered regions of HSPB6 are transformed into well-defined conformations upon the interaction, whereby an unexpectedly asymmetric structure is formed. This structure provides the first atomic resolution snapshot of a human small HSP in functional state, explains how 14-3-3 proteins sequester their regulatory partners, and can inform the design of small-molecule interaction modifiers to be used as myorelaxants.

PubMed: 28089448
DOI: 10.1016/j.str.2016.12.005

実験手法

X-RAY DIFFRACTION (4.5 Å)