5LS8

Light-activated ruthenium complex bound to a DNA quadruplex

Summary for 5LS8

• Entry DOI: 10.2210/pdb5ls8/pdb
• Descriptor: DNA (5'-D(*TP*AP*GP*GP*GP*TP*TP*A)-3'), lambda-[Ru(tap2-dppz-CN)]2+, delta-[Ru(tap2-dppz-CN)]2+, ... (5 entities in total)
• Functional Keywords: dna, quadruplex, chiral recognition, ruthenium
• Biological source: synthetic construct
• Total number of polymer chains: 4
• Total formula weight: 14641.25

Authors

McQuaid, K.T.,Abell, H.,Hall, J.P.,Cardin, C.J. (deposition date: 2016-08-22, release date: 2018-01-17, Last modification date: 2024-01-10)

Primary citation

McQuaid, K.,Abell, H.,Gurung, S.P.,Allan, D.R.,Winter, G.,Sorensen, T.,Cardin, D.J.,Brazier, J.A.,Cardin, C.J.,Hall, J.P.
Structural Studies Reveal Enantiospecific Recognition of a DNA G-Quadruplex by a Ruthenium Polypyridyl Complex.
Angew.Chem.Int.Ed.Engl., 58:9881-9885, 2019

PubMed Abstract: By using X-ray crystallography, we show that the complexes Λ/Δ-[Ru(TAP) (11-CN-dppz)] (TAP=1,4,5,8-tetraazaphenanthrene, dppz=dipyridophenazine) bind DNA G-quadruplex in an enantiospecific manner that parallels the specificity of these complexes with duplex DNA. The Λ complex crystallises with the normally parallel stranded d(TAGGGTTA) tetraplex to give the first such antiparallel strand assembly in which syn-guanosine is adjacent to the complex at the 5' end of the quadruplex core. SRCD measurements confirm that the same conformational switch occurs in solution. The Δ enantiomer, by contrast, is present in the structure but stacked at the ends of the assembly. In addition, we report the structure of Λ-[Ru(phen) (11-CN-dppz)] bound to d(TCGGCGCCGA), a duplex-forming sequence, and use both structural models to provide insight into the motif-specific luminescence response of the isostructural phen analogue enantiomers.

PubMed: 30958918
DOI: 10.1002/anie.201814502

Experimental method

X-RAY DIFFRACTION (1.78 Å)