5LMX

Monomeric RNA polymerase I at 4.9 A resolution

5LMX の概要

• エントリーDOI: 10.2210/pdb5lmx/pdb
• EMDBエントリー: 4088
• 分子名称: DNA-directed RNA polymerase I subunit RPA190, DNA-directed RNA polymerases I, II, and III subunit RPABC5, DNA-directed RNA polymerases I and III subunit RPAC2, ... (15 entities in total)
• 機能のキーワード: multi-protein complex, rna polymerase, monomeric form, transcription
• 由来する生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast); 詳細
• タンパク質・核酸の鎖数: 14
• 化学式量合計: 595788.78

構造登録者

Torreira, E.,Louro, J.A.,Gil-Carton, D.,Gallego, O.,Calvo, O.,Fernandez-Tornero, C. (登録日: 2016-08-02, 公開日: 2017-03-22, 最終更新日: 2024-05-15)

主引用文献

Torreira, E.,Louro, J.A.,Pazos, I.,Gonzalez-Polo, N.,Gil-Carton, D.,Duran, A.G.,Tosi, S.,Gallego, O.,Calvo, O.,Fernandez-Tornero, C.
The dynamic assembly of distinct RNA polymerase I complexes modulates rDNA transcription.
Elife, 6:-, 2017

PubMed Abstract: Cell growth requires synthesis of ribosomal RNA by RNA polymerase I (Pol I). Binding of initiation factor Rrn3 activates Pol I, fostering recruitment to ribosomal DNA promoters. This fundamental process must be precisely regulated to satisfy cell needs at any time. We present in vivo evidence that, when growth is arrested by nutrient deprivation, cells induce rapid clearance of Pol I-Rrn3 complexes, followed by the assembly of inactive Pol I homodimers. This dual repressive mechanism reverts upon nutrient addition, thus restoring cell growth. Moreover, Pol I dimers also form after inhibition of either ribosome biogenesis or protein synthesis. Our mutational analysis, based on the electron cryomicroscopy structures of monomeric Pol I alone and in complex with Rrn3, underscores the central role of subunits A43 and A14 in the regulation of differential Pol I complexes assembly and subsequent promoter association.

PubMed: 28262097
DOI: 10.7554/eLife.20832

実験手法

ELECTRON MICROSCOPY (4.9 Å)