5LMW

Llama nanobody PorM_02

Summary for 5LMW

• Entry DOI: 10.2210/pdb5lmw/pdb
• Related: 5FWO 5LMJ
• Descriptor: Nanobody, GLYCEROL (3 entities in total)
• Functional Keywords: nanobody, porphyromonas gingivalis, type 9 secretion system, immune system
• Biological source: Lama glama (llama)
• Total number of polymer chains: 1
• Total formula weight: 14610.22

Authors

Roche, J.,Gaubert, A.,Leone, P.,Roussel, A. (deposition date: 2016-08-01, release date: 2017-05-03, Last modification date: 2024-01-10)

Primary citation

Duhoo, Y.,Roche, J.,Trinh, T.T.N.,Desmyter, A.,Gaubert, A.,Kellenberger, C.,Cambillau, C.,Roussel, A.,Leone, P.
Camelid nanobodies used as crystallization chaperones for different constructs of PorM, a component of the type IX secretion system from Porphyromonas gingivalis.
Acta Crystallogr F Struct Biol Commun, 73:286-293, 2017

PubMed Abstract: PorM is a membrane protein that is involved in the assembly of the type IX secretion system (T9SS) in Porphyromonas gingivalis, a major bacterial pathogen that is responsible for periodontal disease in humans. In the context of structural studies of PorM to better understand T9SS assembly, four camelid nanobodies were selected, produced and purified, and their specific interaction with the N-terminal or C-terminal part of the periplasmic domain of PorM was investigated. Diffracting crystals were also obtained, and the structures of the four nanobodies were solved by molecular replacement. Furthermore, two nanobodies were used as crystallization chaperones and turned out to be valuable tools in the structure-determination process of the periplasmic domain of PorM.

PubMed: 28471361
DOI: 10.1107/S2053230X17005969

Experimental method

X-RAY DIFFRACTION (1.5 Å)