5LMW
Llama nanobody PorM_02
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-09-09 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.8729 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 65.680, 65.680, 88.802 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.580 - 1.500 |
| R-factor | 0.188 |
| Rwork | 0.188 |
| R-free | 0.19600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4y7m |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.980 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.440 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.047 | |
| Number of reflections | 31805 | |
| <I/σ(I)> | 22 | 1.7 |
| Completeness [%] | 99.9 | 99.4 |
| Redundancy | 6.8 | 6.5 |
| CC(1/2) | 0.627 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293.15 | Tri-sodium Citrate PEG 3000 |
