5LMW

Llama nanobody PorM_02

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-09-09
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.8729
Spacegroup name	P 43 21 2
Unit cell lengths	65.680, 65.680, 88.802
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.580 - 1.500
R-factor	0.188
Rwork	0.188
R-free	0.19600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4y7m
RMSD bond length	0.010
RMSD bond angle	0.980
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.440	1.580
High resolution limit [Å]	1.500	1.500
Rmerge	0.047
Number of reflections	31805
<I/σ(I)>	22	1.7
Completeness [%]	99.9	99.4
Redundancy	6.8	6.5
CC(1/2)		0.627

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293.15	Tri-sodium Citrate PEG 3000