5LGZ

Structure of Photoreduced Pentaerythritol Tetranitrate Reductase

Summary for 5LGZ

• Entry DOI: 10.2210/pdb5lgz/pdb
• Descriptor: Pentaerythritol tetranitrate reductase, ISOPROPYL ALCOHOL, 1-DEOXY-1-(7,8-DIMETHYL-2,4-DIOXO-3,4-DIHYDRO-2H-BENZO[G]PTERIDIN-1-ID-10(5H)-YL)-5-O-PHOSPHONATO-D-RIBITOL, ... (4 entities in total)
• Functional Keywords: petnr, petn reductase, fmn, fnr, photoreduction, oxidoreductase
• Biological source: Enterobacter cloacae
• Total number of polymer chains: 1
• Total formula weight: 39895.47

Authors

Kwon, H.,Smith, O.M.,Moody, P.C.E. (deposition date: 2016-07-08, release date: 2017-02-15, Last modification date: 2024-01-10)

Primary citation

Kwon, H.,Smith, O.,Raven, E.L.,Moody, P.C.
Combining X-ray and neutron crystallography with spectroscopy.
Acta Crystallogr D Struct Biol, 73:141-147, 2017

PubMed Abstract: X-ray protein crystallography has, through the determination of the three-dimensional structures of enzymes and their complexes, been essential to the understanding of biological chemistry. However, as X-rays are scattered by electrons, the technique has difficulty locating the presence and position of H atoms (and cannot locate H ions), knowledge of which is often crucially important for the understanding of enzyme mechanism. Furthermore, X-ray irradiation, through photoelectronic effects, will perturb the redox state in the crystal. By using single-crystal spectrophotometry, reactions taking place in the crystal can be monitored, either to trap intermediates or follow photoreduction during X-ray data collection. By using neutron crystallography, the positions of H atoms can be located, as it is the nuclei rather than the electrons that scatter neutrons, and the scattering length is not determined by the atomic number. Combining the two techniques allows much greater insight into both reaction mechanism and X-ray-induced photoreduction.

PubMed: 28177310
DOI: 10.1107/S2059798316016314

Experimental method

X-RAY DIFFRACTION (1.5 Å)