5LG9
Structure of PfIMP2 (Immune Mapped Protein 2 from Plasmodium falciparum) - an antigenic protein
Summary for 5LG9
| Entry DOI | 10.2210/pdb5lg9/pdb |
| NMR Information | BMRB: 34022 |
| Descriptor | Uncharacterized protein (1 entity in total) |
| Functional Keywords | immune mapped protein, apicomplexan, immunogenic, antigen, immune system |
| Biological source | Plasmodium falciparum (isolate 3D7) |
| Total number of polymer chains | 1 |
| Total formula weight | 17607.48 |
| Authors | Benjamin, S.V.,Matthews, S.J. (deposition date: 2016-07-06, release date: 2016-12-07, Last modification date: 2024-05-15) |
| Primary citation | Jia, Y.,Benjamin, S.,Liu, Q.,Xu, Y.,Dogga, S.K.,Liu, J.,Matthews, S.,Soldati-Favre, D. Toxoplasma gondii immune mapped protein 1 is anchored to the inner leaflet of the plasma membrane and adopts a novel protein fold. Biochim. Biophys. Acta, 1865:208-219, 2016 Cited by PubMed Abstract: The immune mapped protein 1 (IMP1) was first identified as a protective antigen in Eimeria maxima and described as vaccine candidate and invasion factor in Toxoplasma gondii. We show here that TgIMP1 localizes to the inner leaflet of plasma membrane (PM) via dual acylation. Mutations either in the N-terminal myristoylation or palmitoylation sites (G2 and C5) cause relocalization of TgIMP1 to the cytosol. The first 11 amino acids are sufficient for PM targeting and the presence of lysine (K7) is critical. Disruption of TgIMP1 gene by double homologous recombination revealed no invasion defect or any measurable alteration in the lytic cycle of tachyzoites. Following immunization with TgIMP1 DNA vaccine, mice challenged with either wild type or IMP1-ko parasites showed no significant difference in protection. The sequence analysis identified a structured C-terminal domain that is present in a broader family of IMP1-like proteins conserved across the members of Apicomplexa. We present the solution structure of this domain determined from NMR data and describe a new protein fold not seen before. PubMed: 27888074DOI: 10.1016/j.bbapap.2016.11.010 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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