5LER
Structure of the bacterial sex F pilus (13.2 Angstrom rise)
This is a non-PDB format compatible entry.
Summary for 5LER
| Entry DOI | 10.2210/pdb5ler/pdb |
| EMDB information | 4044 |
| Descriptor | Pilin, [(2~{S})-3-[[(2~{R})-2,3-bis(oxidanyl)propoxy]-oxidanyl-phosphoryl]oxy-2-hexadec-9-enoyloxy-propyl] hexadecanoate (2 entities in total) |
| Functional Keywords | f-pilus conjugation type iv secretion system phospholipid, cell adhesion, protein fibril |
| Biological source | Escherichia coli |
| Cellular location | Cell inner membrane; Multi-pass membrane protein: B1VC86 |
| Total number of polymer chains | 75 |
| Total formula weight | 562807.98 |
| Authors | Costa, T.R.D.,Ilangovan, I.,Ukleja, M.,Redzej, A.,Santini, J.M.,Smith, T.K.,Egelman, E.H.,Waksman, G. (deposition date: 2016-06-30, release date: 2016-11-02, Last modification date: 2024-05-15) |
| Primary citation | Costa, T.R.,Ilangovan, A.,Ukleja, M.,Redzej, A.,Santini, J.M.,Smith, T.K.,Egelman, E.H.,Waksman, G. Structure of the Bacterial Sex F Pilus Reveals an Assembly of a Stoichiometric Protein-Phospholipid Complex. Cell, 166:1436-1444.e10, 2016 Cited by PubMed Abstract: Conjugative pili are widespread bacterial appendages that play important roles in horizontal gene transfer, in spread of antibiotic resistance genes, and as sites of phage attachment. Among conjugative pili, the F "sex" pilus encoded by the F plasmid is the best functionally characterized, and it is also historically the most important, as the discovery of F-plasmid-mediated conjugation ushered in the era of molecular biology and genetics. Yet, its structure is unknown. Here, we present atomic models of two F family pili, the F and pED208 pili, generated from cryoelectron microscopy reconstructions at 5.0 and 3.6 Å resolution, respectively. These structures reveal that conjugative pili are assemblies of stoichiometric protein-phospholipid units. We further demonstrate that each pilus type binds preferentially to particular phospholipids. These structures provide the molecular basis for F pilus assembly and also shed light on the remarkable properties of conjugative pili in bacterial secretion and phage infection. PubMed: 27610568DOI: 10.1016/j.cell.2016.08.025 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (5 Å) |
Structure validation
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