5LB8

Crystal structure of human RECQL5 helicase APO form.

5LB8 の概要

• エントリーDOI: 10.2210/pdb5lb8/pdb
• 分子名称: ATP-dependent DNA helicase Q5, ZINC ION (2 entities in total)
• 機能のキーワード: helicase, recq, transcription, dna repair, structural genomics, structural genomics consortium, sgc, hydrolase
• 由来する生物種: Homo sapiens (Human)
• 細胞内の位置: Isoform Beta: Nucleus, nucleoplasm . Isoform Alpha: Cytoplasm . Isoform Gamma: Cytoplasm : O94762
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 115725.11

構造登録者

Newman, J.A.,Aitkenhead, H.,Savitsky, P.,Krojer, T.,von Delft, F.,Arrowsmith, C.H.,Edwards, A.M.,Bountra, C.,Gileadi, O.,Structural Genomics Consortium (SGC) (登録日: 2016-06-15, 公開日: 2016-07-06, 最終更新日: 2024-05-08)

主引用文献

Newman, J.A.,Aitkenhead, H.,Savitsky, P.,Gileadi, O.
Insights into the RecQ helicase mechanism revealed by the structure of the helicase domain of human RECQL5.
Nucleic Acids Res., 45:4231-4243, 2017

PubMed Abstract: RecQ helicases are important maintainers of genome integrity with distinct roles in almost every cellular process requiring access to DNA. RECQL5 is one of five human RecQ proteins and is particularly versatile in this regard, forming protein complexes with a diverse set of cellular partners in order to coordinate its helicase activity to various processes including replication, recombination and DNA repair. In this study, we have determined crystal structures of the core helicase domain of RECQL5 both with and without the nucleotide ADP in two distinctly different ('Open' and 'Closed') conformations. Small angle X-ray scattering studies show that the 'Open' form of the protein predominates in solution and we discuss implications of this with regards to the RECQL5 mechanism and conformational changes. We have measured the ATPase, helicase and DNA binding properties of various RECQL5 constructs and variants and discuss the role of these regions and residues in the various RECQL5 activities. Finally, we have performed a systematic comparison of the RECQL5 structures with other RecQ family structures and based on these comparisons we have constructed a model for the mechano-chemical cycle of the common catalytic core of these helicases.

PubMed: 28100692
DOI: 10.1093/nar/gkw1362

実験手法

X-RAY DIFFRACTION (3.4 Å)