5L9T

Model of human Anaphase-promoting complex/Cyclosome (APC/C-CDH1) with E2 UBE2S poised for polyubiquitination where UBE2S, APC2, and APC11 are modeled into low resolution density

Summary for 5L9T

• Entry DOI: 10.2210/pdb5l9t/pdb
• EMDB information: 3433
• Descriptor: Anaphase-promoting complex subunit 1, Anaphase-promoting complex subunit 10, Anaphase-promoting complex subunit 13, ... (17 entities in total)
• Functional Keywords: ubiquitination, multi-protein complex, cell division, conformational regulation, cell cycle
• Biological source: Homo sapiens (Human); More
• Cellular location: Nucleus : Q9BS18 P30260 Q8NHZ8; Isoform 2: Nucleus. Isoform 3: Cytoplasm: Q9UM11; Bud neck : P34244; Cytoplasm : Q9NYG5 Q96DE5; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome : Q13042
• Total number of polymer chains: 22
• Total formula weight: 1253349.97

Authors

Brown, N.G.,VanderLinden, R.,Dube, P.,Haselbach, D.,Peters, J.M.,Stark, H.,Schulman, B.A. (deposition date: 2016-06-11, release date: 2016-10-26, Last modification date: 2016-11-02)

• Primary citation: Brown, N.G.,VanderLinden, R.,Watson, E.R.,Weissmann, F.,Ordureau, A.,Wu, K.P.,Zhang, W.,Yu, S.,Mercredi, P.Y.,Harrison, J.S.,Davidson, I.F.,Qiao, R.,Lu, Y.,Dube, P.,Brunner, M.R.,Grace, C.R.,Miller, D.J.,Haselbach, D.,Jarvis, M.A.,Yamaguchi, M.,Yanishevski, D.,Petzold, G.,Sidhu, S.S.,Kuhlman, B.,Kirschner, M.W.,Harper, J.W.,Peters, J.M.,Stark, H.,Schulman, B.A.; Dual RING E3 Architectures Regulate Multiubiquitination and Ubiquitin Chain Elongation by APC/C.; Cell, 165:1440-1453, 2016; PubMed: 27259151; DOI: 10.1016/j.cell.2016.05.037

Experimental method

ELECTRON MICROSCOPY (6.4 Å)