Crystal Structure of Human DNA Polymerase Eta Inserting dTMPNPP Opposite O6-Methyl-2'-deoxyguanosine

Summary for 5L1J

Related5L1L 5L1K 5L1I
DescriptorDNA polymerase eta, DNA (5'-D(*CP*AP*TP*(6OG)P*AP*TP*GP*AP*CP*GP*CP*T)-3'), DNA (5'-D(*AP*GP*CP*GP*TP*CP*AP*T)-3'), ... (7 entities in total)
Functional Keywordscatalytic domain, dna damage, dna polymerase, lesion bypass, o6-methyl-2'-deoxyguanosine, y-family polymerase, translesion dna synthesis (tls), dna binding protein., transferase-dna complex, transferase/dna
Biological sourceHomo sapiens (Human)
Cellular locationNucleus  Q9Y253
Total number of polymer chains3
Total molecular weight55342.64
Patra, A.,Egli, M. (deposition date: 2016-07-29, release date: 2016-10-05, Last modification date: 2017-09-20)
Primary citation
Patra, A.,Zhang, Q.,Guengerich, F.P.,Egli, M.
Mechanisms of Insertion of dCTP and dTTP Opposite the DNA Lesion O6-Methyl-2'-deoxyguanosine by Human DNA Polymerase eta.
J.Biol.Chem., 291:24304-24313, 2016
PubMed: 27694439 (PDB entries with the same primary citation)
DOI: 10.1074/jbc.M116.755462
MImport into Mendeley
Experimental method

Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers 0.2104 0.5% 2.4% 4.2%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
Download full validation reportDownload
PDB entries from 2020-10-28