5KVY

CRYSTAL STRUCTURE OF THE TWO TANDEM RRM DOMAINS OF PUF60 BOUND TO A PORTION OF AN ADML PRE-MRNA 3' SPLICE SITE ANALOG

5KVY の概要

• エントリーDOI: 10.2210/pdb5kvy/pdb
• 関連するPDBエントリー: 5KW1 5KW6
• 分子名称: Poly(U)-binding-splicing factor PUF60, DNA (30-MER), CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: tandem rrms, protein-nucleic acid complex, splicing factor, splicing-dna complex, splicing/dna
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 55714.95

構造登録者

Hsiao, H.-H.,Crichlow, G.V.,Albright, R.A.,Murphy, J.W.,Lolis, E.J.,Braddock, D.T. (登録日: 2016-07-15, 公開日: 2017-08-23, 最終更新日: 2023-10-04)

主引用文献

Hsiao, H.T.,Crichlow, G.V.,Murphy, J.W.,Folta-Stogniew, E.J.,Lolis, E.J.,Braddock, D.T.
Unraveling the mechanism of recognition of the 3' splice site of the adenovirus major late promoter intron by the alternative splicing factor PUF60.
Plos One, 15:e0242725-e0242725, 2020

PubMed Abstract: Pre-mRNA splicing is critical for achieving required amounts of a transcript at a given time and for regulating production of encoded protein. A given pre-mRNA may be spliced in many ways, or not at all, giving rise to multiple gene products. Numerous splicing factors are recruited to pre-mRNA splice sites to ensure proper splicing. One such factor, the 60 kDa poly(U)-binding splicing factor (PUF60), is recruited to sites that are not always spliced, but rather function as alternative splice sites. In this study, we characterized the interaction of PUF60 with a splice site from the adenovirus major late promoter (the AdML 3' splice site, AdML3'). We found that the PUF60-AdML3' dissociation constants are in the micromolar range, with the binding affinity predominantly provided by PUF60's two central RNA recognition motifs (RRMs). A 1.95 Å crystal structure of the two PUF60 RRMs in complex with AdML3' revealed a dimeric organization placing two stretches of nucleic acid tracts in opposing directionalities, which can cause looping of nucleic acid and explain how PUF60 affects pre-mRNA geometry to effect splicing. Solution characterization of this complex by light-scattering and UV/Vis spectroscopy suggested a potential 2:1 (PUF602:AdML3') stoichiometry, consistent with the crystal structure. This work defines the sequence specificity of the alternative splicing factor PUF60 at the pre-mRNA 3' splice site. Our observations suggest that control of pre-mRNA directionality is important in the early stage of spliceosome assembly, and advance our understanding of the molecular mechanism by which alternative and constitutive splicing factors differentiate among 3' splice sites.

PubMed: 33253191
DOI: 10.1371/journal.pone.0242725

実験手法

X-RAY DIFFRACTION (1.95 Å)