5KTB

Structure of a complex between S. cerevisiae Csm1 and Mam1

「4EMC」から置き換えられました

5KTB の概要

• エントリーDOI: 10.2210/pdb5ktb/pdb
• 分子名称: Monopolin complex subunit CSM1, Monopolin complex subunit MAM1 (2 entities in total)
• 機能のキーワード: monopolin, replication
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 51595.14

構造登録者

Corbett, K.D.,Harrison, S.C. (登録日: 2016-07-11, 公開日: 2016-07-20, 最終更新日: 2023-10-04)

主引用文献

Corbett, K.D.,Harrison, S.C.
Molecular architecture of the yeast monopolin complex.
Cell Rep, 1:583-589, 2012

PubMed Abstract: The Saccharomyces cerevisiae monopolin complex directs proper chromosome segregation in meiosis I by mediating co-orientation of sister kinetochores on the meiosis I spindle. The monopolin subunits Csm1 and Lrs4 form a V-shaped complex that may directly crosslink sister kinetochores. We report here biochemical characterization of the monopolin complex subunits Mam1 and Hrr25 and of the complete four-protein monopolin complex. By purifying monopolin subcomplexes with different subunit combinations, we have determined the stoichiometry and overall architecture of the full monopolin complex. We have determined the crystal structure of Csm1 bound to a Mam1 fragment, showing how Mam1 wraps around the Csm1 dimer and alters the stoichiometry of kinetochore-protein binding by Csm1. We further show that the kinase activity of Hrr25 is altered by Mam1 binding, and we identify Hrr25 phosphorylation sites on Mam1 that may affect monopolin complex stability and/or kinetochore binding in meiosis.

PubMed: 22813733
DOI: 10.1016/j.celrep.2012.05.012

実験手法

X-RAY DIFFRACTION (3.05 Å)