Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-04-21 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97949 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 101.851, 101.851, 225.077 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.920 - 3.050 |
R-factor | 0.2146 |
Rwork | 0.213 |
R-free | 0.24060 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3n4x |
RMSD bond length | 0.009 |
RMSD bond angle | 1.158 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.220 |
High resolution limit [Å] | 3.050 | 3.050 |
Rmerge | 0.125 | 0.991 |
Number of reflections | 26073 | |
<I/σ(I)> | 5.5 | 1.4 |
Completeness [%] | 98.6 | 99.7 |
Redundancy | 2.3 | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 100 mM HEPES, pH 7.5, 100 mM magnesium chloride, 6% PEG4000 |