Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 101.851, 101.851, 225.077 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.920 - 3.050 |
| R-factor | 0.2146 |
| Rwork | 0.213 |
| R-free | 0.24060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3n4x |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.158 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.220 |
| High resolution limit [Å] | 3.050 | 3.050 |
| Rmerge | 0.125 | 0.991 |
| Number of reflections | 26073 | |
| <I/σ(I)> | 5.5 | 1.4 |
| Completeness [%] | 98.6 | 99.7 |
| Redundancy | 2.3 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 100 mM HEPES, pH 7.5, 100 mM magnesium chloride, 6% PEG4000 |
