5KR4

Directed Evolution of Transaminases By Ancestral Reconstruction. Using Old Proteins for New Chemistries

Summary for 5KR4

• Entry DOI: 10.2210/pdb5kr4/pdb
• Related: 5KQT 5KQU 5KQW 5KR3 5KR5 5KR6
• Descriptor: 4-aminobutyrate transaminase, PYRIDOXAL-5'-PHOSPHATE, 1,2-ETHANEDIOL, ... (4 entities in total)
• Functional Keywords: phylogenetics, directed evolution, transaminase, protein engineering, transferase
• Biological source: Pseudomonas
• Total number of polymer chains: 2
• Total formula weight: 104516.09

Authors

Wilding, M.,Newman, J.,Peat, T.S.,Scott, C. (deposition date: 2016-07-06, release date: 2017-07-12, Last modification date: 2023-10-04)

• Primary citation: Wilding, M.,Peat, T.S.,Kalyaanamoorthy, S.,Newman, J.,Scott, C.,Jermiin, L.S.; Reverse engineering: transaminase biocatalyst development using ancestral sequence reconstruction; Green Chemistry, 19:5375-, 2017; DOI: 10.1039/c7gc02343j

Experimental method

X-RAY DIFFRACTION (2 Å)