5KPE

Solution NMR Structure of Denovo Beta Sheet Design Protein, Northeast Structural Genomics Consortium (NESG) Target OR664

Summary for 5KPE

• Entry DOI: 10.2210/pdb5kpe/pdb
• Related: 5KPH
• NMR Information: BMRB: 30128
• Descriptor: De novo Beta Sheet Design Protein OR664 (1 entity in total)
• Functional Keywords: structural genomics, psi-biology, northeast structural genomics consortium, nesg, de novo protein
• Biological source: synthetic construct
• Total number of polymer chains: 1
• Total formula weight: 13594.00

Authors

Tang, Y.,Liu, G.,Baker, D.,Montelione, G.T.,Northeast Structural Genomics Consortium (NESG) (deposition date: 2016-07-03, release date: 2016-09-21, Last modification date: 2024-05-15)

Primary citation

Marcos, E.,Basanta, B.,Chidyausiku, T.M.,Tang, Y.,Oberdorfer, G.,Liu, G.,Swapna, G.V.,Guan, R.,Silva, D.A.,Dou, J.,Pereira, J.H.,Xiao, R.,Sankaran, B.,Zwart, P.H.,Montelione, G.T.,Baker, D.
Principles for designing proteins with cavities formed by curved beta sheets.
Science, 355:201-206, 2017

PubMed Abstract: Active sites and ligand-binding cavities in native proteins are often formed by curved β sheets, and the ability to control β-sheet curvature would allow design of binding proteins with cavities customized to specific ligands. Toward this end, we investigated the mechanisms controlling β-sheet curvature by studying the geometry of β sheets in naturally occurring protein structures and folding simulations. The principles emerging from this analysis were used to design, de novo, a series of proteins with curved β sheets topped with α helices. Nuclear magnetic resonance and crystal structures of the designs closely match the computational models, showing that β-sheet curvature can be controlled with atomic-level accuracy. Our approach enables the design of proteins with cavities and provides a route to custom design ligand-binding and catalytic sites.

PubMed: 28082595
DOI: 10.1126/science.aah7389

Experimental method

SOLUTION NMR