5KN8

MutY N-terminal domain in complex with undamaged DNA

5KN8 の概要

• エントリーDOI: 10.2210/pdb5kn8/pdb
• 関連するPDBエントリー: 5KN9
• 分子名称: Adenine DNA glycosylase, DNA (5'-D(*AP*TP*CP*CP*TP*GP*TP*GP*CP*T)-3'), DNA (5'-D(*AP*GP*CP*AP*CP*AP*GP*GP*AP*T)-3'), ... (6 entities in total)
• 機能のキーワード: adenine glycosylase, oxog, dna repair protein, lesion-scanning complex, hydrolase-dna complex, hydrolase/dna
• 由来する生物種: Geobacillus stearothermophilus; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 33111.19

構造登録者

Wang, L.,Chakravarthy, S.,Verdine, G.L. (登録日: 2016-06-27, 公開日: 2017-02-08, 最終更新日: 2023-09-27)

主引用文献

Wang, L.,Chakravarthy, S.,Verdine, G.L.
Structural Basis for the Lesion-scanning Mechanism of the MutY DNA Glycosylase.
J. Biol. Chem., 292:5007-5017, 2017

PubMed Abstract: The highly mutagenic A:8-oxoguanine (oxoG) base pair is generated mainly by misreplication of the C:oxoG base pair, the oxidation product of the C:G base pair. The A:oxoG base pair is particularly insidious because neither base in it carries faithful information to direct the repair of the other. The bacterial MutY (MUTYH in humans) adenine DNA glycosylase is able to initiate the repair of A:oxoG by selectively cleaving the A base from the A:oxoG base pair. The difference between faithful repair and wreaking mutagenic havoc on the genome lies in the accurate discrimination between two structurally similar base pairs: A:oxoG and A:T. Here we present two crystal structures of the MutY N-terminal domain in complex with either undamaged DNA or DNA containing an intrahelical lesion. These structures have captured for the first time a DNA glycosylase scanning the genome for a damaged base in the very first stage of lesion recognition and the base extrusion pathway. The mode of interaction observed here has suggested a common lesion-scanning mechanism across the entire helix-hairpin-helix superfamily to which MutY belongs. In addition, small angle X-ray scattering studies together with accompanying biochemical assays have suggested a possible role played by the C-terminal oxoG-recognition domain of MutY in lesion scanning.

PubMed: 28130451
DOI: 10.1074/jbc.M116.757039

実験手法

X-RAY DIFFRACTION (1.81 Å)