5KN8

MutY N-terminal domain in complex with undamaged DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-25
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	49.200, 38.618, 83.796
Unit cell angles	90.00, 104.00, 90.00

Refinement procedure

Resolution	46.354 - 1.810
R-factor	0.1692
Rwork	0.167
R-free	0.21960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1rrq
RMSD bond length	0.009
RMSD bond angle	1.474
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	81.310	1.850
High resolution limit [Å]	1.810	1.810
Rmerge		0.112
Number of reflections	28148
<I/σ(I)>	11.6
Completeness [%]	99.7
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	100 mM Tris pH8.5, 200 mM Ca(OAc)2, 18% (wt/vol) PEG 4000