5KN8
MutY N-terminal domain in complex with undamaged DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-04-25 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97918 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.200, 38.618, 83.796 |
Unit cell angles | 90.00, 104.00, 90.00 |
Refinement procedure
Resolution | 46.354 - 1.810 |
R-factor | 0.1692 |
Rwork | 0.167 |
R-free | 0.21960 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rrq |
RMSD bond length | 0.009 |
RMSD bond angle | 1.474 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 81.310 | 1.850 |
High resolution limit [Å] | 1.810 | 1.810 |
Rmerge | 0.112 | |
Number of reflections | 28148 | |
<I/σ(I)> | 11.6 | |
Completeness [%] | 99.7 | |
Redundancy | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 100 mM Tris pH8.5, 200 mM Ca(OAc)2, 18% (wt/vol) PEG 4000 |