Summary for 5KMZ
| Entry DOI | 10.2210/pdb5kmz/pdb |
| Descriptor | Telomerase RNA pseudoknot (1 entity in total) |
| Functional Keywords | telomerase, rna, pseudoknot, triplex |
| Biological source | Tetrahymena thermophila |
| Total number of polymer chains | 1 |
| Total formula weight | 9888.92 |
| Authors | Cash, D.D.,Feigon, J. (deposition date: 2016-06-27, release date: 2016-10-12, Last modification date: 2024-05-01) |
| Primary citation | Jiang, J.,Chan, H.,Cash, D.D.,Miracco, E.J.,Ogorzalek Loo, R.R.,Upton, H.E.,Cascio, D.,O'Brien Johnson, R.,Collins, K.,Loo, J.A.,Zhou, Z.H.,Feigon, J. Structure of Tetrahymena telomerase reveals previously unknown subunits, functions, and interactions. Science, 350:aab4070-aab4070, 2015 Cited by PubMed Abstract: Telomerase helps maintain telomeres by processive synthesis of telomere repeat DNA at their 3'-ends, using an integral telomerase RNA (TER) and telomerase reverse transcriptase (TERT). We report the cryo-electron microscopy structure of Tetrahymena telomerase at ~9 angstrom resolution. In addition to seven known holoenzyme proteins, we identify two additional proteins that form a complex (TEB) with single-stranded telomere DNA-binding protein Teb1, paralogous to heterotrimeric replication protein A (RPA). The p75-p45-p19 subcomplex is identified as another RPA-related complex, CST (CTC1-STN1-TEN1). This study reveals the paths of TER in the TERT-TER-p65 catalytic core and single-stranded DNA exit; extensive subunit interactions of the TERT essential N-terminal domain, p50, and TEB; and other subunit identities and structures, including p19 and p45C crystal structures. Our findings provide structural and mechanistic insights into telomerase holoenzyme function. PubMed: 26472759DOI: 10.1126/science.aab4070 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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