5KKG

Crystal structure of E72A mutant of ancestral protein ancMT of ADP-dependent sugar kinases family

Summary for 5KKG

• Entry DOI: 10.2210/pdb5kkg/pdb
• Related: 5K27
• Descriptor: ancMT E72A, ADENOSINE MONOPHOSPHATE, IODIDE ION, ... (5 entities in total)
• Functional Keywords: ancestral protein reconstruction, adp-dependent sugar kinases family, glucokinase, phosphofructokinase, bifuncional enzymes, enzyme evolution, de novo protein
• Biological source: synthetic construct
• Total number of polymer chains: 2
• Total formula weight: 110656.68

Authors

Castro-Fernandez, V.,Herrera-Morande, A.,Zamora, R.,Merino, F.,Pereira, H.M.,Brandao-Neto, J.,Garratt, R.,Guixe, V. (deposition date: 2016-06-21, release date: 2017-07-26, Last modification date: 2023-09-27)

• Primary citation: Castro-Fernandez, V.,Herrera-Morande, A.,Zamora, R.,Merino, F.,Gonzalez-Ordenes, F.,Padilla-Salinas, F.,Pereira, H.M.,Brandao-Neto, J.,Garratt, R.C.,Guixe, V.; Reconstructed ancestral enzymes reveal that negative selection drove the evolution of substrate specificity in ADP-dependent kinases.; J. Biol. Chem., 292:21218-21218, 2017; PubMed: 29273585; DOI: 10.1074/jbc.AAC117.001147

Experimental method

X-RAY DIFFRACTION (2.608 Å)