5KJ0

CRYSTAL STRUCTURE OF THE FIRST BROMODOMAIN OF HUMAN BRD4 IN COMPLEX WITH DB-1-264-2

Summary for 5KJ0

• Entry DOI: 10.2210/pdb5kj0/pdb
• Descriptor: Bromodomain-containing protein 4, 4-[[(7~{R})-8-cyclopentyl-7-ethyl-5-methyl-6-oxidanylidene-7~{H}-pteridin-2-yl]-methyl-amino]-3-methoxy-~{N}-(1-methylpiperidin-4-yl)benzamide, 1,2-ETHANEDIOL, ... (4 entities in total)
• Functional Keywords: bromodomain, cap, hunk1, mcap, protein binding-inhibitor complex, mitotic chromosome associated protein, cell cycle, inhibitor, transcription-inhibitor complex, transcription/inhibitor
• Biological source: Homo sapiens (Human)
• Cellular location: Nucleus: O60885
• Total number of polymer chains: 1
• Total formula weight: 16069.54

Authors

Zhu, J.-Y.,Ember, S.W.,Schonbrunn, E. (deposition date: 2016-06-17, release date: 2017-08-09, Last modification date: 2023-09-27)

Primary citation

Koblan, L.W.,Buckley, D.L.,Ott, C.J.,Fitzgerald, M.E.,Ember, S.W.,Zhu, J.Y.,Liu, S.,Roberts, J.M.,Remillard, D.,Vittori, S.,Zhang, W.,Schonbrunn, E.,Bradner, J.E.
Assessment of Bromodomain Target Engagement by a Series of BI2536 Analogues with Miniaturized BET-BRET.
ChemMedChem, 11:2575-2581, 2016

PubMed Abstract: Evaluating the engagement of a small molecule ligand with a protein target in cells provides useful information for chemical probe optimization and pharmaceutical development. While several techniques exist that can be performed in a low-throughput manner, systematic evaluation of large compound libraries remains a challenge. In-cell engagement measurements are especially useful when evaluating compound classes suspected to target multiple cellular factors. In this study we used a bioluminescent resonant energy transfer assay to assess bromodomain engagement by a compound series containing bromodomain- and kinase-biasing polypharmacophores based on the known dual BRD4 bromodomain/PLK1 kinase inhibitor BI2536. With this assay, we discovered several novel agents with bromodomain-selective specificity profiles and cellular activity. Thus, this platform aids in distinguishing molecules whose cellular activity is difficult to assess due to polypharmacologic effects.

PubMed: 27862999
DOI: 10.1002/cmdc.201600502

Experimental method

X-RAY DIFFRACTION (1.51 Å)