5KCS

Cryo-EM structure of the Escherichia coli 70S ribosome in complex with antibiotic Evernimycin, mRNA, TetM and P-site tRNA at 3.9A resolution

This is a large structure.

Summary for 5KCS

Related5KCR
EMDB information8238 8237
Descriptor23S Ribosomal RNA, 5S Ribosomal RNA, 50S ribosomal protein L2, ... (59 entities in total)
Functional Keywordsavilamycin, evernimycin, antibiotic, antimicrobial, ribosome, cryo-em, smfret, rrna, l16, resistance, translation
Biological sourceEscherichia coli (strain K12)
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Total number of polymer chains58
Total molecular weight2302639.41
Authors
Arenz, S.,Juette, M.F.,Graf, M.,Nguyen, F.,Huter, P.,Polikanov, Y.S.,Blanchard, S.C.,Wilson, D.N. (deposition date: 2016-06-06, release date: 2016-08-17, Last modification date: 2018-07-18)
Primary citation
Arenz, S.,Juette, M.F.,Graf, M.,Nguyen, F.,Huter, P.,Polikanov, Y.S.,Blanchard, S.C.,Wilson, D.N.
Structures of the orthosomycin antibiotics avilamycin and evernimicin in complex with the bacterial 70S ribosome.
Proc.Natl.Acad.Sci.USA, 113:7527-7532, 2016
PubMed: 27330110 (PDB entries with the same primary citation)
DOI: 10.1073/pnas.1604790113
MImport into Mendeley
Experimental method
ELECTRON MICROSCOPY
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Structure validation

ClashscoreRamachandran outliersSidechain outliersRNA backbone92.6%0.1%0.60MetricValuePercentile RanksWorseBetterPercentile relative to all structuresPercentile relative to all EM structures

More Asymmetric unit images

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