5K7T

MicroED structure of thermolysin at 2.5 A resolution

5K7T の概要

• エントリーDOI: 10.2210/pdb5k7t/pdb
• 関連するPDBエントリー: 5K7N 5K7O 5K7P 5K7Q 5K7R 5K7S
• EMDBエントリー: 8216 8217 8218 8219 8220 8221 8222
• 分子名称: Thermolysin, CALCIUM ION, ZINC ION, ... (6 entities in total)
• 機能のキーワード: hydrolase
• 由来する生物種: Bacillus thermoproteolyticus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 34724.29

構造登録者

de la Cruz, M.J.,Hattne, J.,Shi, D.,Seidler, P.,Rodriguez, J.,Reyes, F.E.,Sawaya, M.R.,Cascio, D.,Eisenberg, D.,Gonen, T. (登録日: 2016-05-26, 公開日: 2017-04-05, 最終更新日: 2024-02-28)

主引用文献

de la Cruz, M.J.,Hattne, J.,Shi, D.,Seidler, P.,Rodriguez, J.,Reyes, F.E.,Sawaya, M.R.,Cascio, D.,Weiss, S.C.,Kim, S.K.,Hinck, C.S.,Hinck, A.P.,Calero, G.,Eisenberg, D.,Gonen, T.
Atomic-resolution structures from fragmented protein crystals with the cryoEM method MicroED.
Nat. Methods, 14:399-402, 2017

PubMed Abstract: Traditionally, crystallographic analysis of macromolecules has depended on large, well-ordered crystals, which often require significant effort to obtain. Even sizable crystals sometimes suffer from pathologies that render them inappropriate for high-resolution structure determination. Here we show that fragmentation of large, imperfect crystals into microcrystals or nanocrystals can provide a simple path for high-resolution structure determination by the cryoEM method MicroED and potentially by serial femtosecond crystallography.

PubMed: 28192420
DOI: 10.1038/nmeth.4178

実験手法

ELECTRON CRYSTALLOGRAPHY (2.5 Å)